Effects of oppCDF Gene Delection on the Pathogenicity of Salmonella Typhimurium
[Objective]Salmonella is a common zoonotic pathogen that could cause a variety of foodborne diseases,and Salmonella Typhimurium is one of the important serotypes of Salmonella,which had important public health research significance.The purpose of this study was to study the effect of transporter-related gene oppCDF on the biological characteristics of Salmonella Typhimurium,and to provide a theoretical basis for the preparation of a new attenuated vaccine against Salmonella.[Method]In this study,the relationship between oppCDF gene and the biological characteristics of Salmonella Typhimurium was analyzed by comparing the biochemical characteristics,genetic stability,drug resistance,adhesion,invasiveness,half lethal dose(LD50)of Salmonella Typhimurium wild-type strain and the successfully constructed oppCDF gene deletion strains,as well as the bacterial load capacity of mouse liver and spleen.[Result]The results showed that the biochemical characteristics of Salmonella Typhimurium with oppC,oppD and oppF genes deletion did not change significantly and could be inherited stably,indicating that the phenotype of oppCDF gene did not change.Compared with wild-type strain STM LT2,gene deletion strain STM LT2△oppC became susceptible to erythromycin,and the sensitivity of chloramphenicol decreased.STM LT2△oppD was less sensitive to cephalosporin,STM LT2△op pF was more sensitive to compound sulfamethoxazole,and all three gene deletion strains were insensitive to ampicillin.The LD50 of the three gene deletion strains showed that,compared with wild-type strain STM LT2,the virulence of STM LT2△oppC decreased,but the virulence of STM LT2△oppD and STM LT2△oppF increased by 1.6 and 8.6 times,respectively.Compared with wild-type strain STM LT2,the adhesion of STM LT2△oppC was significantly reduced(P＜0.05),the invasiveness was reduced,and the colonization capacity was extremely significantly reduced(P＜0.01).The adhesion and invasiveness of STM LT2△oppD were decreased,but the colonization was extremely significantly increased(P＜0.01).And the adhesion of STM LT2△oppF was significantly decreased(P＜0.05),but the invasiveness and the colonization was extremely significantly increased(P＜0.01).[Conclusion]oppC gene deletion could reduce the pathogenicity of Salmonella Typhimurium,but oppD and oppF genes deletion could enhance the pathogenicity of Salmonella Typhimurium,and the above results provided a theoretical basis for the study of the pathogenicity of Salmonella.
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