Isolation and Identification of a Multidrug-resistant Strain of Enterococcus faecalis from Dog and Analysis of Its Pathogenicity
[Objective]This study was to investigate the pathogenesis of a case of canine genitourinary tract infection.[Method]This study aseptically collected urine from sick dog and identified the bacteria by isolation and purification,Gram staining microscopy,biochemical tests,and 16S rRNA gene amplification,and determined the biological characteristics of the purified strains by drug sensitivity test,virulence gene test,drug resistance gene test,and pathogenicity test of the bacteria.[Result]A parthenogenetic anaerobic Gram-positive coccus was isolated.The colony morphology was like a pinpoint,smooth,translucent,rounded raised,and neatly edged.The biochemical characterization showed that the causative agent was able to ferment lactose,fructose,sucrose,maltose,glucose,sorbitol,mannitol,and arginine,but not xylose,oxidase,citrate,cottonseed sugar,ornithine decarboxylase.The 16S rRNA gene sequence showed greater than 99％similarity to the published sequence of Enterococcus faecalis(E.faecalis),the isolate was identified as E.faecalis,and named EFGZ23GY01.The results of drug sensitivity test showed that this isolate was only sensitive to vancomycin and chloramphenicol,moderately sensitive to neomycin,and resistant to 25 drugs including clindamycin.The results of 8 E.faecalis virulence genes showed that this strain of E.faecalis carried efaA,ace,asa1 and gelE virulence genes.And the results of 14 resistance genes showed that this strain of E.faecalis carried resistance genes,such as tetL,sul Ⅰ,tetC,blaTEM,blaOXA-22,aadA1 and sulⅢ.Pathogenicity test showed that 5 mice showed symptoms such as depression and disorganized coat after 12 h of injection,but did not die,while 1 mouse died after 48 h of injection,indicating that the isolate was pathogenic to mice.[Conclusion]In the present study,a strain of E.faecalis was successfully isolated from canine urine species,which carried multiple virulence and resistance genes and was pathogenic to mice.
Enterococcus faecalis from dogisolation and identificationvirulence genesdrug resistance genespathogenicity
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