Protective Effect of Alcohol Extract from Curcuma kwangsiensis on Lung Injury Induced by LPS in Mice
[Objective]The objective of this study was to investigate the protective effect of alcohol extract from Curcuma kwangsiensis on acute lung injury(ALI)induced by lipopolysaccharide(LPS)in mice.[Method]A total of 54 male Kunming mice were randomly divided into 6 groups:Blank,model,dexamethasone positive control,and alcohol extract from Curcuma kwangsiensis low(2 g/kg),medium(4 g/kg)and high(40 g/kg)groups,with 9 mice in each group.Administration groups were given dexamethasone(5 mg/kg)or corresponding concentration of alcohol extract from Curcuma kwangsiensis(0.2 mL/10 g)by intragastric administration,respectively.Mice in blank and model groups were given the corresponding volume of 0.5％carboxymethyl sodium vitamin(CMC-Na)by intragastric administration for 5 days.1 h after the last administration,except for blank group,mice in other groups were intraperitoneally injected with 10 mg/kg LPS,anesthetized and killed 12 h later,and lung tissue was dissected.During the experiment,the general conditions of mice before and after modeling were observed,the changes of body weight and lung index of mice were counted,and the ratio of wet weight to dry weight of lung was measured.HE staining was used to observe the lung histopathological changes and lung injury score.Real-time quantitative PCR was used to detect the mRNA expression of inflammatory factors,including tumor necrosis factor-alpha(TNF-α),interleukin-6(IL-6),myeloid differentiation factor 88(MyD88),Toll-like receptor 4(TLR4)and nuclear transcription factor κB(NF-κB)in lung.[Result]Compared with blank group,the body weight growth rate of mice in model group was extremely significantly decreased(P＜0.01),the lung index,the ratio of wet weight to dry weight of lung and total lung injury score were extremely significantly increased(P＜0.01),and the mRNA relative expression of related inflammatory factors was significantly or extremely significantly increased(P＜0.05 or P＜0.01),the ALI model was suscessfully constructed.Compared with model group,the body weight growth rate of mice in each dose group of alcohol extract from Curcuma kwangsiensis was significantly or extremely significantly increased(P＜0.05 or P＜0.01).The lung index and the ratio of wet weight to dry weight of lung in medium and high dose groups of alcohol extract from Curcuma kwangsiensis were significantly or extremely significantly decreased(P＜0.05 or P＜0.01).The total lung injury score and the mRNA expression of TNF-α and IL-6 genes in lung of mice in high dose group of alcohol extract from Curcuma kwangsiensis were significantly decreased(P＜0.05).The mRNA expression of NF-κB gene in lung of mice in medium dose group of alcohol extract from Curcuma kwangsiensis was significantly decreased(P＜0.05).[Conclusion]Alcohol extract from Curcuma kwangsiensis had a protective effect on LPS-induced ALI in mice,and its mechanism might be related to the reduction of inflammatory damage mediated by TLR4/MyD88/NF-κB signaling pathway.
alcohol extract from Curcuma kwangsiensislipopolysaccharidemiceacute lung injuryTLR4/MyD88/NF-κB pathway
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