Analysis of Differentially Expressed Genes and Regulatory Pathways in Intramuscular Fat Deposition of Ningxiang Pigs at Different Developmental Stages
[Objective]This study was conducted to screen out differentially expressed genes(DEGs)and their regulatory signaling pathways related to lipid metabolism by analyzing mRNA expression profiles in longissimus dorsi muscle tissues of Ningxiang pigs at different ages,and determine the expression characteristics of some key genes during the development of intramuscular fat.[Method]Ningxiang pigs of 30(D30,n=3)and 250(D250,n=3)days old were selected,the longissimus dorsi muscle tissues were collected to extract total RNA for RNA-Seq,and sequencing data was compared and processed.The DEGs related to lipid metabolism were screened for GO function,KEGG pathway and protein interactions analysis.Six DEGs were randomly selected for Real-time quantitative PCR validation.[Result]A total of 30 DEGs related to lipid metabolism were screened by RNA-Seq,among which 9 genes were up-regulated and 21 genes were down-regulated.The DEGs were mainly enriched in biological process such as fatty acid oxidation and long-chain fatty acid metabolic process;Cellular components such as mitochondrion and organelle membrane;Molecular functions such as acylglycerol O-acy ltransferase activity and oxidoreductase activity.The DEGs were involved signaling pathway such as fatty acid degradation,glycerolipid metabolism and PPAR.Key protein genes such as ACSL1,ACAA2,HADHB,LIPG,GPCPD1 and ALDH2 were screened out by protein interaction analysis,which were involved in the regulation of lipid metabolism through their mRNA expression.Real-time quantitative PCR validation results of six genes such as ACSL1 and CPT1A were consistent with RNA-Seq results.[Conclusion]This study screened some key genes such as ACSL1,ACAA2,HADHB,LIPG,GPCPD1 and ALDH2 participating in the regulation of intramuscular fat in Ningxiang pigs,and played a regulatory role through signaling pathways such as fatty acid degradation and glycerolipid metabolism.The resuts provided a theoretical reference for further research on the molecular regulation of genes related to intramuscular fat deposition in pigs.
万方数据
维普
