Cloning,Molecular Characteristics and Tissue Differential Expression Analysis of KLF15 Gene in Buffalo
[Objective]The aim of this study was to obtain Krüppel-like factor 15(KLF15)gene in buffalo,analyze its molecular characteristics and tissue differential expression,and initially elucidate the structure and function of this gene.[Method]The CDS region of KLF15 gene in buffalo was cloned,and comparative analysis were performed on gene structure,amino acid sequence identity,physicochemical properties,motifs,conserved domains,biological functions,and so on.The expression of KLF15 gene in various tissues of buffalo were detected by Real-time quantitative PCR.[Result]The length of KLF15 gene CDS in buffalo was 1 212 bp,encoding 403 amino acids.Bioinformatics analysis showed that buffalo KLF15 had a high sequence identity with other bovine species,it was a hydrophilic protein in nucleus with no signal peptide or transmembrane helix,which contained conserved domains of the KLF15_N,COG5048 and zf-C2H2 zinc finger structures.Buffalo KLF15 was mainly regulate DNA transcription,bind DNA,bind zinc finger structure.KLF15 gene was most highly expressed in fat of lactating buffalo while in muscle of non-lactating buffalo.In both lactating and non-lactating buffaloes,KLF15 gene was moderately expressed in mammary gland,heart,liver and spleen,while low or almost no expression was observed in lung,kidney,rumen and small intestine.[Conclusion]Buffalo KLF15 was a protein localised in nucleus that binding to zinc finger structures to regulate DNA transcription,which played a role in tissues with vigorous lipid synthesis.
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