Effect of Allium mongolicum Regel Powder on Nutrient Apparent Digestibility and Plasma Metabolomics in Angus Calves
[Objective]The purpose of this experiment was to explore the effects of different levels of Allium mongolicum Regel powder on nutrient apparent digestibility and the characteristics of plasma metabolomics in Angus calves,in order to provide some theoretical basis for the application of Allium mongolicum Regel as a plant additive in Angus calves production.[Method]Twenty-four Angus calves were randomly divided into 4 groups,with 6 calves in each group.The calves in control group(CON)were fed with TMR basal diet,and the calves in experimental groups were fed with Allium mongolicum Regel powder at the dosage of 10(LAMR),15(MAMR)and 20(HAMR)g/d,respectively.The adaptation period was 15 days and the experimental period lasted for 120 days,the experimental period was divided into 2 parts of 60 days each.Feed and fecal samples were collected for 7 days from 114th-120th days of the experimental period to determine the apparent digestibility of nutrients,and blood samples were collected before the morning feeding on the 113th day of the trial period for the determination of plasma non-targeted metabonomics.[Result]Compared with CON group,the apparent digestibility of crude protein,calcium,acidic detergent fiber and neutral detergent fiber of Angus calves in LAMR and MAMR groups were significantly increased(P＜0.05),and the effect of LAMR group was the best.The results of metabonomics showed that 62 differential metabolites were screened out,of which 11 were up-regulated and 30 down-regulated in positive ion mode,and 1 was up-regulated and 20 down-regulated in negative ion mode.These differential metabolites were mainly lipids and lipid-like molecules,organic acids and derivatives and organo-heterocyclic compounds,they mainly regulated the metabolic pathway of alpha-linolenic acid by up-regulating metabolites such as PC 19∶0_20∶3,PC 18∶0_18∶1 and 25-hydroxycholecalciferol,and promoted the utilization of nutrients in Angus calves.[Conclusion]Adding 10 g/d Allium mongolicum Regel powder to the diet had the best effect on the apparent digestibility of nutrients in Angus calves.The active components in Allium mongolicum Regel powder might regulate the pathway of alpha-linolenic acid metabolic by up-regulating the metabolites such as glycerophospholipid compounds(PC 19∶0_20∶3,PC 18∶0_18∶1)and 25-hydroxycholecalciferol,thus affecting the utilization of nutrients in Angus calves.
Allium mongolicum Regel powderAngus calvesnutrient apparent digestibilitymetabolomicsactive element of plant
万方数据
维普
