Protective Effect of Selenomethionine on Spleen Injury Induced by Aflatoxin B1 in Rabbits
[Objective]To investigate the protective effect of selenomethionine(SeMet)on spleen injury induced by aflatoxin B1(AFB1)in rabbits.[Method]50 rabbits aged 35 days were randomly divided into 5 groups,10 in each group,namely control group(basal diet),model group(0.3 mg/kg BW AFB,+basal diet),Se-1 group(0.2 mg/kg SeMet+0.3 mg/kg BW AFB1+basal diet),Se-2 group(0.4 mg/kg SeMet+0.3 mg/kg BW AFB,+basal diet)and Se-3 group(0.6 mg/kg SeMet+0.3 mg/kg BW AFB,+basal diet).The experimental period was 21 days.After the experiment,the spleen was taken out to observe the morphological changes of spleen tissue by HE staining,PCNA protein expression by immunohistochemical staining,apoptosis rate by TUNEL staining,and the oxidative stress level and inflammatory factors content in spleen tissue were detected.[Result]Compared with control group,the red pulp and white pulp of the spleen of rabbits in model group were arranged disorder,the number of lymphocytes decreased obviously,PCNA protein expression decreased and a large number of apoptotic cells appeared.Compared with model group,SeMet supplementation in diet could significantly improve the pathological damage of spleen induced by AFB1 poisoning in rabbits.At the same time,SeMet could also increase the activities of total antioxidant capacity(T-AOC),superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in spleen tissue(P＜0.01),and significantly reduce the contents of malondialdehyde(MDA),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and IL-1β(P＜0.01).[Conclusion]SeMet supplementation could effectively improve AFB1-induced spleen injury in rabbits by increasing the activity of antioxidant enzymes and decreasing the expression of inflammatory factors.Among them,0.2 mg/kg SeMet had the best response.
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