In response to the demands of social and economic growth,the pig industry is steadily transitioning towards large-scale,intensive and highly efficient breeding methods.Artificial insemination(AI)is currently used in pig reproduction as one of the most effective biological technology,in which the preservation of high-quality boar semen is a key step in artificial insemination.The quality of pig semen after preservation directly affects the fertility rate of sows and the survival rate of piglets,and its quality will be influenced by various factors such as temperature,pH,microorganisms and semen extenders during semen preservation.Nowadays,three methods of pig semen preservation are mainly adopted in production practice:Room temperature preservation,low temperature preservation and cryopreservation.Room temperature preservation utilizes the acidic metabolites produced by the semen at 15-25 ℃ to achieve short-term preservation by acid inhibition.Low temperature preservation involves slowly cooling the semen to 0-5 ℃ under the protection of cryoprotectants to preserve the sperm by keeping it in a semi-dormant state.Cryopreservation utilizes cryogenic sources such as liquid nitrogen to rapidly freeze the semen to-196 to-79 ℃,thereby inhibiting physiological metabolic activities of sperm to achieve long-term preservation of pig semen.The authors aim to comprehensively review three distinct methods of pig semen preservation technology,provide a concise overview of the primary factors that impact the efficacy of this preservation process,and strive to present improvement strategies tailored to each preservation method,so as to contribute valuable references and insights to the research and implementation of artificial insemination technology,as well as the preservation of porcine germplasm resources within China's pig industry.Ultimately,its objective is to facilitate the sustainable and healthy growth of China's pig industry.
pigssemen preservationroom temperature preservationlow temperature preservationcryopreservation
万方数据
维普
