Transcriptome Construction of F3 Follicle and Analysis of Genes Related to Follicle Atresia in Pigeons
[Objective]This study was aimed to screen key genes involved in follicular atresia by high-throughput sequencing of granulosa cell of F3 follicle in pigeons.[Method]Three White King pigeons at laiying interval 5(LI5)and 7(LI7)days were selected to construct the transcriptome library of granulosa cell of F3 follicle,respectively,and RNA-Seq was used to screen and annotate the differentially expressed genes(DEGs),the expression of 5 randomly selected DEGs were verified by Real-time quantitative PCR.[Result]The clean reads of 41.96-43.62 Mb were obtained from 6 cDNA libraries of granulosa cells of F3 follicle in pigeons at LI5 and LI7 stages,the value of Q30 was greater than 92％,and the comparison rate was ranged from 82.21％to 85.94％.A total of 6 587 DEGs were obtained from granulosa cell of F3 follicle in pigeons at LI5 and LI7 stages,of which 2 318 DEGs were up-regulated and 4 269 were down-regulated.GO function enrichment analysis results of DEGs was performed,mainly in important biological pathways such as cell process,metabolic process,binding and catalytic activity.KEGG pathway enrichment analysis results showed that DEGs were mainly involved in focal adhesion,protein processing in endoplasmic reticulum,ovarian steroidogenesis and steroid hormone biosynthesis.Combining protein-protein interaction analysis and key DEGs in critical pathways,it found that StAR,HSD3B1,MARCH6,BCL2,BOK and CDCA7 genes played an important role in follicular atresia in pigeons.Real-time quantitative PCR results showed that the expression trends of 5 genes in granulosa cells of F3 follicle in pigeons at LI5 and LI7 stages were consistent with the results of RNA-Seq.[Conclusion]Six key genes(StAR,HSD3B1,MARCH6,BCL2,BOK and CDCA7)were obtained,which played an important role in follicular atresia in pigeons.The results provided a basis for further understanding of the molecular mechanism of follicular atresia and a theoretical basis for inhibiting follicular atresia and improving egg production in pigeons.
万方数据
维普
