Isolation,Identification and Genome Sequence Analysis of Two Recombinant Strains of Avian Infectious Bronchitis Virus
[Objective]The experiment was aimed to understand the genetic variation of avian Infectious bronchitis virus(IBV).[Method]Samples suspected to be infected with IBV was collected from partial farms in Shandong,inoculated with SPF chicken embryos for virus isolation and identification.Whole genome sequencing was performed using second-generation sequencing technology,and genetic evolution and key amino acid sites were analyzed based on the whole genome sequence.[Result]Autopsy of infected chickens showed hemorrhages in the tracheal rings and yellow or black-yellow embolism in the lumen.Two strains of IBV were isolated from the samples,and named SDJN and SDLY,respectively.Similarity analysis showed that isolates SDJN and SDLY had high similarity with G Ⅰ-19 genotype reference strains,with nucleotide similarity of 96.0％-97.3％and amino acid similarity of 94.9％-100％.Amino acid sequence analysis showed that the two IBV isolates had multiple amino acid variation sites compared with other strains.Phylogenetic tree results showed that isolates SDJN and SDLY were located in the same branch as the reference strains of the IBV G Ⅰ-19 genotype.Recombinant analysis showed that isolates SDJN and SDLY were recombinant strains of multiple genotypic strains,both with the ck/CH/LDL/091022 strain as the main parental strain.[Conclusion]In this study,two IBV isolates were isolated from Shandong province.The two isolates were closely related to GⅠ-19 genotype,and were a new recombinant IBV strain.The results laid a foundation for further study on the prevalence and evolution of IBV and the prevention and control of avian infectious bronchitis.
万方数据
维普
