Study on Bacterial Flora Changes in Producing Bedding Process Using Cow Manure by Anaerobic Fermentation Technology in Dairy Farms
[Objective]The changes of bacterial flora were investigated for producing bedding process using cow manure by anaerobic fermentation technology in dairy farms,in order to provide some theoretical references for standardized production,the reasonable and safe use of cow manure as bedding.[Method]Samples were collected from four large-scale dairy farms,including cow manure before anaerobic fermentation,cow manure after solid-liquid separation during anaerobic fermentation,and cow manure bedding on the bed.Bacterial DNA was extracted for high-throughput 16S rRNA sequencing,and the microbial diversity,composition,and significant differences in characteristic microbial communities at different stages were analyzed.[Result]Compared with cow manure before fermentation,the diversity and richness of microbial communities in cow manure after anaerobic fermentation solid-liquid separation were reduced,but were similar to those in cow manure substrate.The phyla and genus with the highest abundance in cow manure before fermentation were Firmicutes(55.15％)and UCG-005(16.68％),respectively.The phyla and genus with the highest abundance in cow manure after anaerobic fermentation solid-liquid separation were Firmicutes(48.12％)and Bacillus(12.16％),respectively.The phyla and genus with the highest abundance in cow manure bedding were Actinobacteriota(37.02％)and Glutamicibacter(11.65％),respectively.LEfSe analysis showed that there were a total of 14 bacterial genera with significant differences between the three groups of cow manure before fermentation,cow manure after anaerobic fermentation solid-liquid separation,and bedding.[Conclusion]Through the study of the microbial community composition during anaerobic fermentation of cow manure production in dairy farms,it was found that the dominant bacterial phyla and genus had undergone significant changes.After fermentation treatment,the diversity and richness of the microbial community had decreased.This production process could meet the safety requirements of dairy farms for cow manure production.
万方数据
维普
