Target Gene Prediction and Bioinformatics Analysis of miR-141 in Capra hircus
[Objective]This study was aimed to explore the regulatory mechanism of miR-141 in glucolipid metabolism and bone metabolism,and provide a basis for establishing animal model related to diabetes mellitus with osteoporosis and researching the regulation mechanism of miR-141 related gene expression.[Method]Mature miR-141 sequences of different species were obtained from miRbase database for sequence alignment and conservative analysis used BioEdit and WebLogo softwares.The PROMO and JASPAR online softwares were used to predict the transcription factor binding site of miR-141 in Capra hircus.The target genes of intersection were predicted by TargetScan and miRwalk online tools.GO function and KEGG pathway enrichment analysis of the predicted target genes were performed by DAVID and KOBAS online softwares.The functional protein interaction was analyzed with Networkanalyst database,meanwhile drawing the network regulation diagram.The YM500V2 online database was used to analyze the expression of miR-141 target genes in different tissues of Capra hircus.[Result]miR-141 mature sequences among different species were highly conservative.There were many transcription factor binding sites such as C/EBPα,MyoD,YY1 and Sp1 in the promoter region of miR-141 in Capra hircus.A total number of 89 target genes included Nfatc2,Egr2 and Fasl were predicted using different databases.GO function enrichment analysis results showed that the predicted target genes of miR-141 mainly enriched in the regulation of transcription from RNA polymerase Ⅱ promoter and regulation of transcription-DNA-templated for biological process,nucleus and cytoplasm for cellular component,protein binding and DNA binding for molecular function.KEGG pathway enrichment analysis results showed that the target genes were significantly enriched in regulation of actin cytoskeleton,tight junction,axon guidance,Hedgehog signaling pathway,cGMP-PKG signaling pathway,microRNA in cancer and other signaling pathways.miR-141 target genes interaction analysis results revealed that Nfatc2 and Egr2 genes were connected by Fasl gene,which had more targeted relationships with other proteins,and involved in several signaling pathways,such as TGF-β,Hedgehog,Wnt/β-catenin and other pathways.miR-141 was expressed in different tissues of Capra hircus,and its expression exhibited tissue differences.[Conclusion]miR-141 participated in regulating some signaling pathways,represented by Hedgehog and Wnt/β-catenin signaling pathways with target genes Nfatc2,Egr2 and Fasl,and further regulated the glucolipid metabolism as well as physiological and biochemical reactions of musculoskeletal system in Capra hircus.
万方数据
维普
