Effect of Different Concentrations of Ellagic Acid on Preservation of Pig Semen at Room Temperature
[Objective]The aim of this study was to investigate the effects of ellagic acid(EA)on the quality and antioxidant properties of boar semen during room temperature preservation,in order to provide a theoretical basis for the promotion and application of EA in semen preservation.[Method]Semen was collected from four healthy adult Duroc boars aged 2 to 3 years.The qualified semen was divided into five portions and diluted with BTS(Beltsville thawing solution)diluted at room temperature,which contained different concentrations of EA(0(control),5,10,15 and 20 μg/mL).Sperm motility,average path velocity,swing amplitude,whipping frequency,plasma membrane integrity rate,acrosome integrity rate,and antioxidant capacity were assessed on the 1st,3rd,5th,and 7th days of preservation.[Result]On the 1st day of preservation,plasma membrane integrity was significantly higher in 15 μg/mL EA group compared to control group(P＜0.05),and there was no significant difference in other indicators among experimental groups and control group(P＞0.05).On the 3rd day of semen preservation,the average path velocity,catalase(CAT)activity,and total antioxidant capacity(T-AOC)of sperm in 10 and 15 μg/mL EA groups were significantly higher than those in control group(P＜0.05).On the 5th day of semen preservation,the swing amplitude of sperm in 15 and 20 μg/mL EA groups was significantly lower than that in control group and the 5 and 10 μg/mL EA groups(P＜0.05),and the sperm motility in 5,10 and 15 μg/mL EA groups was significantly higher than that in control group(P＜0.05).The CAT,glutathione peroxidase(GSH-Px)activitives,and T-AOC in the semen of 10,15 and 20 μg/mL EA groups were significantly higher than those in control group(P＜0.05).On the 7th day of semen preservation,the sperm motility,average path velocity,plasma membrane integrity,acrosome integrity,CAT activity,and T-AOC in each experimental group were significantly higher than those in control group(P＜0.05).[Conclusion]The incorporation of EA into BTS dilution solution was found to offer a protective effect on semen during room-temperature storage,with the optimal outcome observed at an EA concentration of 10 μg/mL.
ellagic acidroom temperature preservationsemen qualityantioxidant abilitypig
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