Study on the Structure and Function of the Archaea Community and Its Correlation with Diarrhea of Weaned Piglets
[Objective]The aim of this experiment was to study the structure and function of Archaea community in intestine of weaned piglets,and analyze the correlation between Archaea and diarrhoea in weaned piglets,so as to provide theoretical basis for the prevention and treatment of post-weaning piglet diarrhea.[Method]Thirty-two healthy weaned piglets(H group)and thirty-two diarrheal weaned piglets(D group)at 24 days of age were selected from the same batch.Fecal samples were collected and the structure of Archaea community of the fecal samples from both groups was analyzed using 16S rRNA gene sequencing.PICRUSt2 software was used to predict the function of Archaea community in intestine,and the correlation between differential Archaea and diarrhea in weaned piglets was analyzed.[Result]There was significant difference of diarrhea scores between healthy and diarrheal weaned piglets(P＜0.05).Euryarchaeota,Crenarchaeota and Thaumarchaeota were the dominant Archaea phyla in healthy and diarrheal weaned piglets.The dominant genera in healthy weaned piglets were Methanosarcina,Euryarchaeota_norank and Methanobrevibacter,while the dominant genera in diarrheal weaned piglets were Euryarchaeota_norank,Methanosarcina and Methanobrevibacter.The diversity and richness of Archaea community in diarrheal weaned piglets was higher than those in healthy weaned piglets.There were significant difference in the structure and function of the Archaeal community between healthy and diarrheal weaned piglets(P＜0.05).The difference in the structure of Archaea community between healthy and diarrheal weaned piglets were mainly reflected in the genus Methanosarcina,Euryarchaeota_norank,Sul fophobococcus,Nitrosarchaeum,Pyrodictium,etc.The difference in the function of Archaea community primarily existed in lipopolysaccharide biosynthesis,biosynthesis of amino acids,antigen processing and presentation,etc.The results of correlation analysis indicated that the relative abundance of Methanosarcina,Sul fophobococcus,Euryarchaeota_norank,Methanocaldococcus,Pyrococcus,Methanocalculus were significantly correlated with diarrhea in weaned piglets(P＜0.05).[Conclusion]There was significant difference in the structure and function of Archaea community between healthy and diarrheal weaned piglets.The relative abundance of Euryarchaeota_norank was higher in diarrheal weaned piglets,while the relative abundance of Methanosarcina was lower.There was a correlation between Euryarchaeota_norank,Methanosarcina,Sulfophobococcus and diarrhea in weaned piglets.
万方数据
维普
