Effects of Alkaloids from Sophora alopecuroides on Growth Performance and Serum Biochemical Indexes of Dumeng Lambs Fed with High Concentrate Diet
[Objective]This experiment was conducted to investigate the effects of Sophora alopecuroides alkaloids on growth performance and blood indexes of Dumeng lambs fed with high-concentrate diet.[Method]Thirty-two Dumeng lambs with body weight of(25.76±2.35)kg were randomly divided into 4 groups with 8 lambs in each group.The basal diet of each experimental group was the same,which was a high-concentrate diet with a concentrate to forage ratio of 7∶3,and the lambs in control group were fed with the basal diet.The lambs in group Ⅰwere fed with the diet added 0.2%Sophora alopecuroides,in group Ⅱ were fed with the diet supplemented with 0.121 g/kg Sophora alopecuroides alkaloids(low-dose alkali group),and in group Ⅲ were fed with the diet suplemented with 0.169 g/kg Sophora alopecuroides alkaloids(high-dose alkali group).The pre-feeding period was 15 days,and the feeding period was 60 days.The lambs were fed in a single cage and fed ad libitum.During the experiment,the daily feed intake of each sheep was recorded.The lambs were weighed with an empty stomach on days 0,30 and 60 of the experiment to calculate growth performance indicators.After weighing on the 60th day,blood was collected from the jugular vein of the lambs for measurement of serum biochemical index.[Result]The average daily gain of lambs in groups Ⅰ and Ⅱ were significantly higher than that in control group and group Ⅲ from 0 to 30 d(P<0.05).From 30 to 60 d,the average daily gain and daily feed intake of lambs in group Ⅱ were significantly higher than those in group Ⅲ(P<0.05).From 0 to 60 d,the average daily gain of lambs in group Ⅱ was significantly higher than that in group Ⅲ(P<0.05).On the 60 th day of the experiment,compared with group Ⅲ,the activities of serum aspartate aminotransferase(AST)and alanine aminotransferase(ALT)in groups Ⅰ and Ⅱ were significantly decreased(P<0.05),and the contents of total protein(TP)and albumin(ALB)in serum of lambs in group Ⅱ were significantly increased(P<0.05).Compared with control group,the serum glucose(GLU)contents of groups Ⅰ,Ⅱ and Ⅲ were significantly decreased(P<0.05),the content of serum immunoglobulin M(IgM)in group Ⅱwas extremely significantly increased(P<0.01),and the content of serum IgA in group Ⅱ was significantly higher than that in group m(P<0.05).[Conclusion]The addition of 0.121 g/kg Sophora alopecuroides alkaloids under high-concentrate diet could improve the growth performance of Dumeng lambs,alleviate liver damage,reduce GLU content and regulate humoral immune function.