Comparison of Follicle Development,Antioxidant Capacity and Autophagy Level Between Shan-ma Duck at Peak and Late Stages of Laying
[Objective]To investigate the changes of follicle development,gonadotropin levels,antioxidant capacity and follicle autophagy related genes in ducks at different laying stages,and to lay a research foundation for developing new technology to prolong laying period of laying birds.[Method]The single factor completely randomized experimental design was adopted,and 84 normal laying ducks(Longyan Shan-ma duck)in the peak laying stage(25 weeks)and the late laying stage(70 weeks)were selected.These ducks were randomly divided into 6 replicates,with 14 ducks in each replicate.The ducks were fed with the same corn-soybean meal basal diet for 8 weeks.At the end of the 8th week,3 healthy and normal laying ducks were randomly selected from each replicate for blood collection and slaughter sampling to detect blood biochemical indicators and ovarian development indicators.[Result]Compared with the duck in peak laying stage,①The number of small yellow follicles,the index of small yellow follicles and the average weight of small yellow follicles of laying duck in late laying stage were significantly reduced,and the weight of atretic follicles was significantly increased(P＜0.05).②The plasma follicle-stimulating hormone(FSH)level of laying duck in late laying stage was significantly lower(P＜0.05),the activities of glutathione peroxidase(GSH-Px)and total superoxide dismutase(T-SOD)in small yellow follicles and the malondialdehyde(MDA)level were significantly higher,and the activities of GSH-Px and T-SOD in atretic follicles were significantly lower(P＜0.05).③The mRNA expression level of follicle-stimulating hormone receptor(FSHR)in follicles of laying duck in late laying stage was reduced,and forkhead transcription factor 1(FOXO1)was activated and regulated autophagy-related genes(selective autophagy receptor(SQSTM1)and beclin 1(BECN1))(P＜0.05).[Conclusion]When Shan-ma ducks entered the late laying period,the content and sensitivity of FSH in follicles were decreased due to the increase of oxidative stress,and FOXOl was activated to regulate the autophagy of follicular granulosa cells and inhibit follicular development.
万方数据
维普
