Metabolomics Analysis of Fermented Elephant Grass and It's Effects on the Growth Performance and Serum Biochemical Indicators of Pigs
[Objective]To comprehensive analysis the nutritional value of fermented elephant grass used as feed stock for pigs.[Method]Gas chromatography-mass spectrometry(GC-MS)was used to comparatively analysis the metabolites of fermented elephant grass.The different levels of fermented elephant grass mixed with cassava and fish sauce were added into the basal diet for pigs.Pigs in control group were fed with basal diet,in groups 1,Ⅱ,m,Ⅳ,Ⅴ and Ⅵ were fed with test diets supplemented by 0,2％,3％,4％,6％and 9％fermented elephant grass.The feeding period was 28 days.After the feeding experiment,the growth performance of pigs were measured,and blood was collected for measuring serum biochemical indicators.[Result]①There were 121 different metabolites in fermented elephant grass with 92 differential metabolites up-regulated.Most of them were sugars,amino acids sand fatty acids.Among them,the levels of tricose,L-aspartic acid,glutamate,inositol,methionine and N-acetyl-ornithine were increased more than 1 000 times.There were 11 extremely significantly different metabolic pathways between elephant grass and fermented elephant grass(P＜0.01).②There were no significant differences in serum biochemical indicators of pigs between the experimental groups and control group(P＞0.05),except for the levels of total cholesterol(TC)and low-density lipoprotein cholesterol(LDLC)in group Ⅱ which were significantly higher than control group(P＜0.05).The levels/activities of total protein(TP),TC,LDLC,albumin(ALB),alanine transaminase(ALT),triglycerides(TG),aspartate aminotransferase(AST)and high-density lipoprotein cholesterol(HDLC)in group Ⅱ were significantly higher than those in other groups(P＜0.05).In the feeding time,the diarrhea rate of pigs in all seven groups were 0.[Conclusion]The nutritional value of fermented elephant grass had been significantly enhanced with significant increases in amino acids,carbohydrates and lipids.Fermented elephant grass could partially replace the basic daily feed for pigs and significantly reduce the fat deposition in pig.Adding 2％fermented elephant grass to diet could get the best comprehensive production efficiency.
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