Effect of Trichomonas on the Structure of Macaca mulatta Gut Microbiota Based on 16S rDNA Sequencing Technology
[Objective]This experiment was aimed to study the changes and diversity of gut microbiota in rhesus monkeys(Macaca mulatta)infected with Trichomonas using 16S rDNA sequencing technology,provide a reference for clinical research on using microbiota as an alternative antibiotics for treating trichomoniasis.[Method]Six samples of rhesus monkeys infected with Trichomonas and six samples of Trichomonas-negative fresh feces from rhesus monkeys were collected for predictive analysis of gut microbiota and functional genes through metagenomic sequencing.[Result]T he diversity of gut microbiota in Trichomonas-infected rhesus monkeys decreased significantly compared to healthy rhesus monkeys,leading to significant changes in microbial community composition.Specifically,there was a significant decrease in Bacteroidota and Fibrobacterota(P＜0.05),while Campylobacta and Desulfobacterota showed a significant increase(P＜0.05).Additionally,Lactobacillales,Campylobacter and Campylobacteraceae were significantly enriched in the intestinal microbes of Trichomonass-infected rhesus monkeys,whereas Prevotella_9,Bacteroidales and Bacteroidota were significantly enriched in the intestinal microbes of Trichomonas-negative rhesus monkeys.Furthermore,Brachyspira_hampsonii and Prevotellaceae_bacterium_Marseille_P2831 were identified as biomarkers of Trichomonas-infected rhesus monkeys.The functional gene prediction of gut microbiota also revealed a significant decrease in the biosynthesis function of amino acids and antibiotics in the Trichomonas-infected rhesus monkeys(P＜0.05).[Conclusion]Infection with Trichomonas could lead to a reduction in the diversity of gut microbiota and an alteration in the composition of the microbial community in rhesus monkeys.Additionally,it could also decrease the biosynthetic function of gut microbiota.
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