Study on the Prevention and Treatment of Fermented Compound Traditional Chinese Medicine of Pleuron ostreatus HXS-M1 on Escherichia coli-induced Diarrhea in Mice
[Objective]The objective of this experiment was to study the clinical therapeutic effect of fermented compound traditional Chinese medicine of Pleuron ostreatus HXS-M1 on Escherichia coli-induced diarrhea in mice,and provide a new therapeutic method for clinical Escherichia coli diarrhea.[Method]The diarrhea model of mice was established by intraperitoneal injection of Escherichia coli suspension(0.2 mL/mouse).40 mice with diarrhea were randomly divided into model group,antibiotic group(AB),traditional Chinese medicine group(TCM)and fermented traditional Chinese medicine group(FTCM),with 10 mice in each group.Another 10 healthy mice were assigned to the blank group with intraperitoneal injection of normal saline(0.2 mL/mouse).Mice in AB group were given enrofloxacin solution(50 mg/mL),mice in TCM group were given traditional Chinese medicine extract(1.0 g/mL),mice in FTCM group were given fermented traditional Chinese medicine extract(1.0 g/mL),mice in model and blank groups were given normal saline intragastric administration once a day(0.2 mL/mouse).The drug was given by continuous intragastric administration for 6 days.The diarrhea,body weight and feed intake of mice were recorded daily.The heart,liver,kidney,spleen and lung tissues of mice were collected 12 h after the last administration,weighed and organ coefficients were calculated.The intestinal contents of mice were collected for colony counting.[Result]The mouse model of Escherichia coli diarrhea was successfully constructed.Compared with blank group,the diarrhea rate of model group was 100％,body weight,average daily gain,average daily feed intake and intestinal lactobacillus number were extremely significantly decreased(P＜0.01),liver index,spleen index and intestinal Escherichia coli and Salmonella number were extremely significantly increased(P＜0.01).Compared with model group,the diarrhea rate of AB and TCM groups was 30％,and the diarrhea rate of FTCM group was 0.In AB group,the final body weight,average daily gain and average daily feed intake were extremely significantly increased(P＜0.01),while liver index,spleen index and the number of Escherichia coli and Salmonella in intestinal were extremely significantly decreased(P＜0.01).The final body weight,average daily gain,average daily feed intake and intestinal lactobacillus quantity in TCM and FTCM groups were extremely significantly increased(P＜0.01),while the ratio of feed to gain,liver index and spleen index,intestinal Escherichia coli and Salmonella quantity were extremely significantly decreased(P＜0.01).[Conclusion]The fermented compound traditional Chinese medicine of Pleurotus ostreatus HXS-M1 could reduce the diarrhea rate,increase the average daily feed intake and average daily gain,reduce the liver index and spleen index,and the number of Escherichia coli and Salmonella in intestine,and increase the number of lactic acid bacteria,so as to have a synergistic protective effect on Escherichia coli diarrhea in mice.The results provided reference for the development of prevention and treatment of diarrhea caused by Escherichia coli infection.
Pleurotus ostreatuscompound traditional Chinese medicineEscherichia colidiarrhea
万方数据
维普
