Isolation,Identification and Detection of Drug Resistance and Virulence Genes of Salmonella Pullorum from a Breeding Chicken Farm of Guangdong
[Objective]The purpose of this experiment was to understand the infection of Salmonella Pullorum in chickens of a breeding farm,and analyze the drug resistance and virulence genes,in order to provide evidence for the prevention and control of pullorosis.[Method]The liver tissue of sick chickens suspected to be suffering from pullorosis was collected and Salmonella was isolated and cultured.The isolates were identified by PCR amplification using specific primers.Molecular typing was obtained by multilocus sequence typing(MLST)technique.The minimum inhibitory concentration of the isolates was determined by agar dilution method.Next-generation sequencing(NGS)technology was utilized to analyze the drug resistance and virulence genes.[Result]A total of 5 isolates of Salmonella Pullorum were isolated and named SPNH05-1 to SPNH05-5,respectively.MLST typing results showed that all the 5 isolates were ST92.The results of drug sensitivity test showed that the isolates were multi-drug resistant,and there were 2 drug resistance profiles,ampicillin+nalidixic acid+sulfamisoxazole and ampicillin+nalidixic acid+sulfamisoxazole+polycolistin B.NGS was performed on isolates SPNH05-1 and SPNH05-2,which showed that there were 28 drug resistance genes,including β-lactam,quinolones,aminoglycosides,sulfonamides and polypeptides,and 151 virulence genes,which were mainly related to Salmonella adhesion,type Ⅲ secretion system,stress and toxin.[Conclusion]The results of this study showed that multi-drug resistant Salmonella Pullorum infection existed in this breeding farm,and the isolates carried multiple drug resistance and virulence genes.The results provided reference for the purification of pullorosis.
Salmonella Pullorumisolation and identificationdrug resistancevirulence gene
万方数据
维普
