Whole Genome Sequencing of Bacteriocin-producing Bacillus velezensis CM7-4 and Its Bacteriostatic Activity
[Objective]Bacillus velezensis CM7-4 was isolated from seawater and had been demonstrated to possess good antibacterial activity.This study was to investigate the antimicrobial mechanism of Bacillus velezensis CM7-4 and analyze the whole genome sequence in order to explore its antibacterial and other functional genes.[Method]The bacteriocin crude extract of Bacillus velezensis CM7-4 was obtained by acid precipitation,and the effect of proteinase K on its antibacterial effect was determined by the solid agar perforation method.Subsequently,the complete genome of Bacillus velezensis CM7-4 was sequenced on the MGI DNBSEQ-T7 platform.Their bacteriocin production potential was analyzed by sequence assembly,gene prediction and functional annotation.[Result]The bacteriocin crude extract of Bacillus velezensis CM7-4 demonstrated a notable bacteriostatic effect on a diverse range of 8 kinds of Gram-negative and positive bacteria,including Escherichia coli,Salmonella,Enterococcus faecalis,Listeria monocytogenes and others.Compared with control group,the bacteriocin crude extract demonstrated a extremely significantly diminished inhibitory effect on the eight indicator bacteria following treatment with proteinase K(P＜0.01).Whole genome sequencing revealed that the genome size of Bacillus velezensis CM7-4 was 3 953 124 bp with a 47.04％GC content.A total of 3 870 protein-coding genes,9 rRNA,81 tRNA and 1 ncRNA were identified.And 11 bacteriocin synthesis genes,10 antioxidant activity genes,and 4 immune and inflammatory signalling pathway regulation genes were predicted.[Conclusion]The results of this experiment indicated that Bacillus velezensis CM7-4 was a potential bacteriocin-producing strain.This study provided a theoretical basis for further study of its antibacterial properties.
Bacilluswhole genome sequencingbacteriocingene function annotation
万方数据
维普
