Study on the Interaction Between Dihydrotestosterone and Melatonin of Hair Follicle Cells in Yak
[Objective]This study was aimed to reveal the interaction effect between dihydrotestosterone(DHT)and melatonin(MLT)of hair follicle cells in yak,so as to provide a basis for elucidating the regulatory mechanism of the two hormones in the development of hair follicle cycle and their application in hair trait selection of yak.[Method]Using the dermal papilla cells(DPCs)and hair matrix cells(HMCs)of yak as research subjects,the effects of different concentrations of DHT(0,0.01,0.1,1,10,20,40 and 80 μmol/L)and MLT(0,250,500,600,750,1 000 and 1 500 pg/mL)on the proliferation of DPCs and HMCs in yak were determined by MTT assay.The concentrations of DHT and MLT that had the most significant effect on hair follicle cells were selected for combined treatment of HMCs and their interaction effect of the two hormones in cell proliferation was detected using MTT method.The test result was further verified by EdU method.The expression of several genes related to cell proliferation and hair follicle development were detected by Real-time quantitative PCR after combined treatment of DHT and MLT,and analyze the underlying mechanisms.[Result]Compared with control group(0 μmol/L),DHT concentrations ranging from 10 to 40 μmol/L could significantly or extremely significantly promote the proliferation of DPCs(P＜0.05 or P＜0.01),and the concentration of DHT at 40-80 μmol/L could extremely significantly promote the proliferation of HMCs(P＜0.01).40 μmol/L DHT was the optimal concentration to promote the proliferation of DPCs and HMCs,while low concentration of DHT(1 μmol/L)could extremely significantly inhibit the proliferation of HMCs(P＜0.01).Compared with control group(0 pg/mL),500 pg/mL MLT had the most significant proliferative effect on DPCs(P＜0.01),while all concentrations of MLT had no significant effect on the proliferation of HMCs(P＞0.05).When HMCs were combining treated with 40 μmol/L DHT and 500 pg/mL MLT for 72 h,compared with DHT group,DHT+MLT extremely significantly reduced the proliferative effect of HMCs(P＜0.01).Real-time quantitative PCR results showed that compared with control group,the expression of LEF1,SFRP1,TGFB2 and BCL2 genes related to hair follicle development were extremely significantly or significantly changed after DHT treatment of HMCs(P＜0.01 or P＜0.05),indicating that DHT might be involve in the regulation of hair follicle development by affecting of these genes.[Conclusion]DPCs and HMCs of yak had different sensitivities to DHT and MLT.A certain concentration of DHT could significantly promote the proliferation of both DPCs and HMCs,while a low concentration of DHT could inhibit the proliferation of HMCs.500 pg/mL MLT had the most significant effect on the proliferation of DPCs,and MLT had no significant effect on HMCs at different concentrations.Finally,MLT might have a function of anti-DHT in HMCs.
万方数据
维普
