Effects of Grape Seed Proanthocyanidins on Growth Performance,Serum Biochemical Indices,and Rumen Environment of Beef Cattle
[Objective]This experiment was conducted to investigate the effects of different dietary levels of grape seed procyanidins(GSPs)on growth performance,nutrient digestion,serum biochemical indices and rumen environment of beef cattle.[Method]Twenty Simmental bulls aged 12 months with good health and body weight of about 400 kg were randomly divided into 4 treatment groups with 5 replicates per group.The cattle in control group was fed a basal diet,in experimental groups was fed the basal diets supplemented with 20 mg/kg(20 GSPs group),40 mg/kg(40 GSPs group)and 80 mg/kg GSPs(80 GSPs group),respectively.The pre-feeding period was 15 days,and the normal test period was 55 days.The initial body weight and final body weight of each cattle were measured,and the average daily feed intake and feed to gain ratio were recorded.Three days before the end of experiment,the nutrient digestibility was measured using the full fecal collection method.After the experiment,15 mL of blood and 50 mL of rumen fluid were collected from each cattle for the determination of serum biochemical indexes,rumen fermentation parameters and rumen 16S rRNA gene sequence.[Result]Compared with control group,①the final weight of Simmental cattle in 20 GSPs and 40 GSPs groups were significantly increased(P＜0.05).The apparent digestibility of dry matter and neutral detergent fiber in 40 GSPs group were significantly enhanced(P＜0.05),and crude protein apparent digestibility in 20 GSPs and 40 GSPs groups were significantly improved(P＜0.05).②The activities of serum aspartate aminotransferase and alanine aminotransferase in 40 GSPs group were remarkably decreased(P＜0.05),serum urea nitrogen contents in all groups supplemented with GSPs were significantly decreased(P＜0.05).③The total antioxidant capacity in 40 GSPs group was significantly increased(P＜0.05),the serum glutathione peroxidase activity in 40 GSPs and 80 GSPs groups were significantly improved(P＜0.05).④ Serum immunoglobulin G and complement 3 contents in 80 GSPs group were significantly increased(P＜0.05).The serum complement 3 and complement 4 contents were significantly increased(P＜0.05),while the serum interleukin-6 was significantly reduced(P＜0.05)in 40 GSPs group.The serum tumor necrosis factor was significantly decreased in 80 GSPs group(P＜0.05).⑤The propionic acid content of 40 GSPs and 80 GSPs groups were significantly enhanced(P＜0.05),the bacterial Chaol index in 20 GSPs group were significantly increased(P＜0.05).The relative abundance of Spirochaetes and Clostridium in 20 GSPs group were significantly increased(P＜0.05).The relative abundance of Fibrobacteres,Actinobacteria and Spirochaetes in 80 GSPs group were significantly increased(P＜0.05),the relative abundance of Succiniclasticum in 40 GSPs and 80 GSPs groups were significantly decreased(P＜0.05).[Conclusion]The supplementation of 40 mg/kg GSPs could increase the final body weight,serum immune and antioxidant levels and rumen bacterial diversity,and improve the composition of rumen microbial community of Simmental cattle.
万方数据
维普
