Effects of Dietary Probiotics and Acid Preparations on Growth Performance and Intestinal Flora of Weaned Piglets
[Objective]The aim of this experiment was to study the effects of adding probiotics and acid preparations to diets on growth performance and intestinal flora of weaned piglets.[Method]120 healthy weaned piglets from the same batch,with good health and of similar weight were selected and randomly divided into 3 groups with 5 replicates of 8 piglets each.The piglets in control group(group A)were fed with basic feed normally.The piglets in groups B and C were fed with basic diet supplemented with 1 g/kg of acid preparation and 2 g/kg of probiotics,respectively.The experimental period was 30 d.[Result]①Compared with group A,the final weight,average daily feed intake(ADFI)and average daily gain(ADG)of piglets in groups B and C were extremely significantly increased(P＜0.01),while the feed to gain ratio(F/G)of piglets in group C was significantly reduced(P＜0.05).②The analysis of intestinal flora diversity based on 16S rDNA sequencing showed that there was no significant difference among the three groups in Alpha diversity,but in Beta diversity,there was significant difference in gut microbiota structure of piglets between group A and groups B and C.In faecal microbial composition,the dominant phyla in the three groups were mainly Firmicutes and Bacteroidetes,and the relative abundance of different phyla in each group showed different trends,with the relative abundance of Bacteroidetes being extremely significantly higher in group B compared to group A(P＜0.01)and that of Spirochaetes was significantly lower in groups B and C compared to group A(P＜0.05).Compared to group A,the relative abundance of Prevotella was found to be significantly or extremely significantly higher in groups B and C(P＜0.05 or P＜0.01),while the relative abundance of Treponema and SMB53 was significantly or extremely significantly lower(P＜0.05 or P＜0.01).The relative abundance of Faecalibacterium and Roseburia was found to be significantly or extremely significantly higher in group C compared to group A(P＜0.05 or P＜0.01),whereas the relative abundance of Oscillospira was significantly lower in group B compared to group A(P＜0.05).③A functional analysis of metabolic pathways in KEGG intergroup colonies revealed that retinol metabolism(ko00830)and ubiquinone and other terpene-quinone biosynthesis(ko00130)pathways were significantly up-regulated in group B compared to group A(P＜0.05),toluene degradation(ko00623)and sphingolipid metabolism(ko00600)were extremely significantly upregulated in group C(P＜0.01).Compared with group B,the degradation of chlorinated alkanes and degradation of chloro-olefins(ko00625)was significantly up-regulated in group C(P＜0.05).[Conclusion]The addition of 2 g/kg probiotics or 1 g/kg acid preparations to the diets of weaned piglets could improve their growth performance,which might be closely related to the addition of probiotics and acidifiers to regulate the intestinal flora.Among them,adding probiotics had a better effect than adding acid preparations.
万方数据
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