Screening of Genes Related to Lactation in Kazakh Horses Based on Transcriptomic
[Objective]The aim of this experiment was to screen the genes affecting lactation in Kazakh horses and provide data support for the selection of Kazakh horses for dairy type.[Method]Transcriptome sequencing was performed on whole blood from the jugular vein of 12 Kazakh horses at peak lactation to screen differentially expressed gene(DEG)related to lactation in Kazakh horses.GO function and KEGG pathway enrichment analysis were performed on DEGs,and Sankey bubble maps of the enriched pathways were plotted to screen for genes related to lactation in Kazakh horses and construct a gene interaction network.6 DEGs were randomly selected for Real-time quantitative PCR to verify the accuracy of transcriptome data.[Result]A total of 286 DEGs were identified in the high and low lactation group,including 198 up-regulated genes and 98 down-regulated genes.27 terms were enriched by GO enrichment analysis,which included cell structure,cellular enzyme activity,substance transport,etc.13 pathways were enriched by KEGG enrichment analysis,which included cellular processes related to cellular autophagy,proliferation and migration as well as neural signaling,hormone secretion,protein metabolism,environmental adaptation and immune diseases.A regulatory network of 95 DEGs was constructed by STRING program,and a Hub group of 10 DEGs was screened out.Real-time quantitative PCR results showed that the trends of the detected genes in the high and low lactation groups of Kazakh horses were basically consistent with RNA-Seq results.[Conclusion]This study screened the genes KCNN4,CAMK2B,CACNA1D,CACNA1E and GRIA4 to be related to the amount of lactation of Kazakh horses during peak lactation,and these genes regulated lactation of Kazakh horses through reciprocal regulation of transmembrane transport,insulin and cortisol secretion and ErbB signaling pathway.
万方数据
维普
