Investigation of the population diversity of intermediate host snails and crabs of Paragonimus along Jiulong River,Zhangjiang River and Dongxi River basins in southern Fujian Province
Objective To investigate the population distribution of intermediate host snails and crabs of Paragonimus along the Jiulongjiang River,Zhangjiang River,and Dongxi River basins in Bopingling Mountain,southern Fujian Province,so as to provide baseline data for researches on parasitic disease prevention and control and enlargement of samples in the parasitic re-source bank.Methods A total of 23 villages in 8 counties(districts)along the Jiulong River,Zhangjiang River,and Dongxi Riv-er basins in Zhangzhou City,Fujian Province were selected as survey sites during the period from November 2020 through March 2023,and snail and freshwater crabs were sampled from 1 to 3 streams and ditches neighboring residential areas in each village.Morphological identification of snails was performed according to the external morphological characteristics of collected snail shells,and the unidentified snail species sampled from the natural foci of paragonimiasis in Yunxiao County were subjected to se-quence analysis of the mitochondrial cytochrome oxidase 1(CO1)gene.The crab species was identified by observing the morpho-logical characteristics of the terminal segment of the first pleopod of male crabs,and Paragonimus cercariae and metacercariae were detected in collected snails.Results The shells of the unidentified snails sampled from the natural foci of paragonimiasis in Yunxiao County were approximately 50 mm in height and 18 mm in width,thick and solid,long tower cone-shaped,and had 8 to 10 whorls.CO1 gene sequence analysis identified the snail species as Sulcospira hainanensis.A total of 6 freshwater snail spe-cies belonging to 5 genera within 3 families,identified 23 survey sites,including Semisulcospira libertina,Paludomus zhangchouensis and S.hainanensis that belonged to the Family Pleurceridae,Tricula fujianensis and T.huaanensis that belonged to the subfamily Triculinae,Family Pomatiopsidae,and Melanoides tuberculata(Family Thiaridae),and 11 species of freshwater crabs belonging to 5 genera within 2 families were identified,including Sinopotamon genus of S.jianglense,S.pinheense,and S.zhangzhouense,Huananpotamon genus of H.planopodum and H.zhangzhouense,Nanhaipotamon genus of N.huaanense and N.longhaiense,and Minpotamon genus of M.nasicum and M.auritum that belonged to the Family Potamidae,and Somanniathel-phusa genus of S.huaanensis and S.zhangpuensis(Family Parathelphusidae).In addition,the prevalence of P.westermani cercari-ae infections was 0.08%(2/2 317)in P.zhangchouensis from Danyan Village in Changtai District and 0.09%(1/1 039)in S.hain-anensis from Jinkeng Village in Yunxiao County,and the prevalence of P.westermani metacercariae infections was 25.81%(8/31)in S.jianglense from Danyan Village in Changtai District,and 26.31%(5/19)in S.zhangzhouense from Jinkeng Village in Yunx-iao County,respectively.Conclusions There is a population diversity in the intermediate host snails and crabs along the Ji-ulongjiang River,Zhangjiang River,and Dongxi River basins in Bopingling Mountain,southern Fujian Province,and P.zhang-zhouensis and S.hainanensis are,for the first time,confirmed as the first intermediate hosts of P.westermani.
ParagonimusParagonimus westermaniIntermediate hostFreshwater snailFreshwater crabPopulation diversi-tyFujian Province
NETL
NSTL
万方数据
