Comparison of the microbiota diversity between autogenous and anautoge-nous Culex pipiens pallens
Objective To investigate the microbiota composition and diversity between autogenous and anautogenous Culex pipiens pallens,so as to provide insights into unraveling the pathogenesis of autogeny in Cx.pipiens pallens.Methods Autoge-nous and anautogenous adult Cx.pipiens pallens samples were collected at 25℃,and the hypervariable regions of the microbial 16S ribosomal RNA(16S rRNA)gene was sequenced on the Illumina NovaSeq 6000 sequencing platform.The microbiota abun-dance and diversity were evaluated using the alpha diversity index,and the difference in the microbiota structure was examined using the beta diversity index.The microbiota with significant differences in the abundance between autogenous and anautoge-nous adult Cx.pipiens pallens samples was identified using the linear discriminant analysis effect size(LEfSe).Results The mi-crobiota in autogenous and anautogenous Cx.pipiens pallens samples belonged to 18 phyla,28 classes,70 orders,113 families,and 170 genera,and the dominant phyla included Proteobacteria,Bacteroidetes,and so on.At the genus level,Wolbachia was a common dominant genus,and the relative abundance was(77.6±11.3)%in autogenous Cx.pipiens pallens samples and(47.5±8.5)%in anautogenous mosquito samples,while Faecalibaculum(0.4%±0.1%),Dubosiella(0.5%±0.0%)and Massilia(0.5%±0.1%)were specific species in autogenous Cx.pipiens pallens samples.Alpha diversity analysis showed that higher Chao1 index and ACE index in autogenous Cx.pipiens pallens samples than in anautogenous samples(both P values>0.05),and lower Shan-non index(P>0.05)and Simpson index(P<0.05)in autogenous Cx.pipiens pallens samples than in anautogenous samples.LEfSe analysis showed a total of 48 significantly different taxa between autogenous and anautogenous Cx.pipiens pallens samples(all P values<0.05).Conclusion There is a significant difference in the microbiota diversity between autogenous and anautoge-nous Cx.pipiens pallens.
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