摘要
目的 了解贵州省六盘水市部分地区蜱携带立克次体感染率,为当地蜱传立克次体病防控工作提供参考依据.方法 2023年4-6月在贵州省六盘水市盘州市大山镇高兴村捕捉牛、羊体表寄生蜱,采用形态学和分子生物学方法对蜱种进行鉴定.采用巢式PCR法检测蜱携带的立克次体,包括斑点热群立克次体、柯克斯体属、无形体属、埃立克体属和东方体属,扩增的阳性片段测序后与GenBank已知序列进行同源性比对.结果 共采集200只蜱,经鉴定均为微小扇头蜱.巢式PCR联合测序鉴定出蜱携带敬信立克次体暂定种(40.50%)、贝氏柯克斯体(1.50%)和柯克斯体共生菌(27.00%),未检测出无形体、埃立克体及东方体.结论 贵州省六盘水市部分地区微小扇头蜱携带敬信立克次体暂定种、贝氏柯克斯体和柯克斯体共生菌;当地有关部门需加强家畜和人群蜱传立克次体感染监测,减少蜱传立克次体病危害.
Abstract
Objective To investigate the prevalence of tick-borne rickettsial infections in selected areas of Liupanshui City,Guizhou Province,2023,so as to provide insights into the management of tick-borne rickettsioses in the city.Methods Ticks were captured from the body surface of bovines and sheep in Gaoxing Village,Dashan Township,Liupanshui City,Guizhou Prov-ince during the period between April and June,2023,and tick species were identified using morphological and molecular biologi-cal techniques.In addition,tick-borne Rickettsia was identified using a nested PCR assay,including spotted fever group rickettsi-ae(SFGR),Coxiella spp.,Anaplasma spp.,Ehrlichia spp.,and Orientia spp.,and positive amplified fragments were sequenced and aligned with known sequences accessed in the GenBank database.Results A total of 200 ticks were collected and all tick species were identified as Rhipicephalus microplus.Nestle PCR assay combined with sequencing identified ticks carrying Candi-datus Rickettsia jingxinensis(40.50%),Coxiella burnetii(1.50%),and Coxiella-like endosymbionts(27.00%),and Anaplasma spp.,Ehrlichia spp.or Orientsia spp.was not detected.Conclusions R.microplus carried Candidatus R.jingxinensis,C.bur-netii,and Coxiella-like endosymbionts in selected areas of Liupanshui City,Guizhou Province.Intensified monitoring of tick-borne rickettsial infections is needed in livestock and humans to reduce the damages caused by rickettsioses.
基金项目
国家自然科学基金(32000139)
病原微生物生物安全国家重点实验室开放研究项目(SKLPBS2217)
NETL
NSTL
万方数据