Investigation of tick-borne Rickettsia in selected areas of Liupanshui City,Guizhou Province in 2023
Objective To investigate the prevalence of tick-borne rickettsial infections in selected areas of Liupanshui City,Guizhou Province,2023,so as to provide insights into the management of tick-borne rickettsioses in the city.Methods Ticks were captured from the body surface of bovines and sheep in Gaoxing Village,Dashan Township,Liupanshui City,Guizhou Prov-ince during the period between April and June,2023,and tick species were identified using morphological and molecular biologi-cal techniques.In addition,tick-borne Rickettsia was identified using a nested PCR assay,including spotted fever group rickettsi-ae(SFGR),Coxiella spp.,Anaplasma spp.,Ehrlichia spp.,and Orientia spp.,and positive amplified fragments were sequenced and aligned with known sequences accessed in the GenBank database.Results A total of 200 ticks were collected and all tick species were identified as Rhipicephalus microplus.Nestle PCR assay combined with sequencing identified ticks carrying Candi-datus Rickettsia jingxinensis(40.50%),Coxiella burnetii(1.50%),and Coxiella-like endosymbionts(27.00%),and Anaplasma spp.,Ehrlichia spp.or Orientsia spp.was not detected.Conclusions R.microplus carried Candidatus R.jingxinensis,C.bur-netii,and Coxiella-like endosymbionts in selected areas of Liupanshui City,Guizhou Province.Intensified monitoring of tick-borne rickettsial infections is needed in livestock and humans to reduce the damages caused by rickettsioses.
Rhipicephalus microplusRickettsiaCandidatus Rickettsia jingxinensisCoxiella burnetiiCoxiella-like endosymbi-ontLiupanshui City
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