宏基因组二代测序技术辅助诊断内脏利什曼病1例
Auxiliary diagnosis of visceral leishmaniasis with metagenomic next-genera-tion sequencing:a case report
李维苹 1王兴蕾 2马丽 3豆欣蔓 4赵睿 3王颖俏1
作者信息
- 1. 兰州大学护理学院(甘肃兰州 730000)
- 2. 兰州大学护理学院(甘肃兰州 730000);兰州大学第二医院肝病科(甘肃兰州 730030)
- 3. 兰州大学第二医院肝病科(甘肃兰州 730030)
- 4. 兰州大学护理学院(甘肃兰州 730000);兰州大学第二医院护理部
- 折叠
摘要
1例患者因"反复高热、肝脾肿大、伴全血细胞减少待查"收住入院,排除细菌、病毒、真菌感染及血液系统疾病后,采集患者外周血行宏基因组二代测序,结果显示婴儿利什曼原虫感染.rk39快速诊断试剂检测显示抗利什曼原虫抗体阳性,骨髓涂片检出利什曼原虫无鞭毛体,最终确诊为内脏利什曼病.给予葡萄糖酸锑钠、激素进行抗感染、保肝、升白细胞等治疗及个性化护理后,患者康复出院.宏基因组测序技术在不明原因发热患者病因检测中具有重要价值,值得临床推广应用.
Abstract
This case report summarizes the experience from diagnosis and treatment of a patient with repeated high fever,hep-atosplenomegaly and pancytopenia.Following exclusion of bacterial,viral,fungal infections and hematological diseases,metage-nomic next-generation sequencing of the patient's peripheral blood revealed Leishmania infantum infection,and rK39 rapid diag-nostic test showed positive for anti-Leishmania antibody,while microscopic examination of bone marrow smears identified Leish-mania amastigotes.Therefore,the case was definitively diagnosed as visceral leishmaniasis,and given anti-infective treatment with sodium antimony gluconate and hormone,hepatoprotection,elevation of white blood cell counts and personalized nursing.Then,the case was cured and discharged from hospital.Metagenomic next-generation sequencing is of great value in etiological detection of fever patients with unknown causes,which deserves widespread clinical applications.
关键词
内脏利什曼病/宏基因组二代测序/辅助诊断/病因检测Key words
Visceral leishmaniasis/Metagenomic next-generation sequencing/Auxiliary diagnosis/Etiological detection引用本文复制引用
基金项目
甘肃省自然科学基金软科学专项(23JRZA483)
甘肃省卫生健康行业科研项目(GSWSHL2022-05)
出版年
2024
NETL
NSTL
万方数据