成簇规律间隔短回文重复序列(clustered regularly interspaced short palindromic repeats,CRISPR)及其相关蛋白(CRISPR-associated protein,Cas)系统(CRISPR/Cas系统)为具有规律簇状短回文重复序列结构的适应性免疫系统,能干扰外源性核酸,保护原核生物免受外部侵害,是一种有效的基因编辑及核酸检测工具.CRISPR/Cas系统在病毒和细菌领域应用广泛,但在寄生虫病领域研究相对较少.本文分类探讨了 CRISPR/Cas系统的作用机制,全面综述了其在寄生虫基因编辑与核酸检测中的应用,旨在为未来寄生虫病相关研究提供参考.
Application of the CRISPR/Cas system in gene editing and nucleic acid de-tection of parasitic diseases:a review
CRISPR/Cas system,an adaptive immune system with clustered regularly interspaced short palindromic repeats,may interfere with exogenous nucleic acids and protect prokaryotes from external damages,is an effective gene editing and nucle-ic acid detection tools.The CRISPR/Cas system has been widely applied in virology and bacteriology;however,there is relatively less knowledge about the application of the CRISPR/Cas system in parasitic diseases.The review summarizes the mechanisms of action of the CRISPR/Cas system and provides a comprehensive overview of their application in gene editing and nucleic acid de-tection of parasitic diseases,so as to provide insights into future studies on parasitic diseases.
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