Prevalence and genetic characteristics of Cryptosporidium infections among HIV-positive individuals in Jiangxi Province
Objective To investigate the prevalence of Cryptosporidium infection and the distribution of parasite species and genotypes among HIV-positive individuals in Jiangxi Province.Methods HIV-positive individuals'sociodemographic and clini-cal data were collected from three AIDS designated hospitals in Jiangxi Province from January 2022 to March 2023.Subjects'stool samples were collected,and genomic DNA was extracted from stool samples.Nested PCR assay was performed based on the small subunit ribosomal RNA(SSU rRNA)gene of Cryptosporidium,and Cryptosporidium gp60 gene was amplified in stool sam-ples positive for the SSU rRNA gene.The second-round PCR amplification product was checked with 1.5%agarose gel electro-phoresis,and the products of suspected positive amplifications were sequenced,followed by sequence alignment.The phylogenet-ic tree was created using the Neighbor-Joining method with the software MEGA 11.0,to characterize the species,genotypes and sub-genotypes of Cryptosporidium.Results A total of 382 HIV-positive individuals were enrolled,with two cases identified with Cryptosporidium infection(0.52%prevalence),and both cases had no abdominal pain or diarrhea.Following sequencing and sequence alignment,the gene sequences of these two Cryptosporidium isolates shared 99.76%and 99.88%similarity with the gene sequence of C.meleagridis isolates.Phylogenetic analysis based on the Cryptosporidium SSU rRNA gene sequence identified the species of these two Cryptosporidium-positive stool samples as C.meleagridis.Following nested PCR amplification of the Crypto-sporidium gp60gene,sequencing and sequence alignment,the two C.meleagridisisolates were characterized asⅢeA17G2R1 andⅢbA25G1R1a sub-genotypes,and the sub-genotypeⅢbA25G1R1a was firstly described in humans.Conclusion The preva-lence of Cryptosporidium is low among HIV-positive individuals in Jiangxi Province.The likelihood of Cryptosporidium infection cannot be neglected among HIV-positive individuals without diarrhea.
CryptosporidiumHIVGenetic characteristicJiangxi Province
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