Differences in gut microbiota among primary school students with different levels of sugar-sweetened beverage consump-tion
Objective To explore the differences in the gut microbiota of primary school students with different levels of sugar-sweetened beverage intake,so as to provide scientific evidence for better identification of health risks in children and the develop-ment of targeted health policies.Methods In June 2022,a total of 192 healthy primary school students from Chengdu were select-ed using a stratified cluster random sampling method.The sugar-sweetened beverage intake was assessed through a dietary frequency questionnaire.Based on the median daily sugar-sweetened beverage intake,primary school students were categorized into a low-in-take group(n=96)and a high-intake group(n=96).The gut microbiota in fresh fecal samples from the two groups of primary school students was analyzed using 16S rRNA high-throughput sequencing,and the diversity and community structure differences in the gut microbiota were compared.Results Children in the low-intake group had a sugar-sweetened beverage intake of(21.3±1.6)mL/d,while the high-intake group had an intake of(269.6±37.3)mL/d.Diversity analysis results showed that there were no statis-tically significant differences between the low-intake and the high-intake group in terms of α diversity metrics:Observed_otus index[298.50(259.75,342.25),305.50(244.25,367.75)],Goods_coverage index[1.00(1.00,1.00),1.00(1.00,1.00)],Chao index[304.18(260.75,348.78),305.88(245.68,370.88)],Shannon index[5.88(5.29,6.45),5.71(4.89,6.28)]and Simp-son index[0.95(0.91,0.97),0.94(0.88,0.97)](Z=-0.64,-0.76,-0.54,-1.76,-1.67,P＞0.05).Furthermore,no statis-tically significant difference was observed in β diversity between the two groups(R2=0.006,P＞0.05).At the genus level,the a-bundance of Blautia[0.033(0.018,0.055)]and Fusicatenibacter[0.009(0.005,0.015)]were higher in the low-intake group compared to the high-intake group[0.024(0.013,0.041),0.006(0.003,0.011)]and differences were statistically significant(Z=-2.52,-2.81,P＜0.05).LEfSe analysis highlighted intergroup differences primarily in Blautia,Fusicatenibacter and Sarcina(LDA=3.56,3.12,3.53,P＜0.05).Conclusions There is no significant difference in the diversity and overall structure of the gut microbiota in primary school students with different levels of sugar-sweetened beverage intake.However,there are species variations at the genus level.The information can serve as a scientific basis for identifying health risks in primary school students and formula-ting targeted health strategies.
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万方数据
