Objective To explore the effects of different screen content on the nighttime sleep quality of college students based on screen time 1 h before bedtime,so as to provide a basis for healthy sleep habits.Methods From March 1 to 15,2024,40 col-lege students were recruited from Capital Institute of Physical Education through student social media groups or recruitment posters,and were randomly assigned to TV/movie streaming group(n=10),video game group(n=10),online surfing/social networking group(n=10),sleep aid video group(n=10)by single-blind randomized experimental design.The International Physical Activity Questionnaire Short Form(IPAQ-SF),Self-rating Depression Scale(SDS)and Self-rating Anxiety Scale(SAS)were used to meas-ure the physical activity and emotional state levels of college students before the experiment.In the 5-day experiment,participants watched different screen content 1 h before sleep,then Pittsburgh Sleep Quality Index(PSQI),sleep duration,sleep latency and wake-up time were collected before and after the intervention.A paired sample t-test was used to compare the differences within groups before and after the intervention,and one-way analysis of variance was used for horizontal comparison between groups.Re-sults In the longitudinal within-group comparisons before and after the intervention,there was an increase in PSQI scores in the TV/movie strearming(5.40±0.70,6.30±0.67)and online surfing/social networking(5.50±0.97,6.20±0.63)groups(t=-3.86,-2.33,P<0.05)and the sleep aid video group(5.80±0.92,4.90±0.74)with a decrease in PSQI scores(t=3.25,P<0.05),and the difference in PSQI scores in the video game group(5.60±0.84,5.80±0.79)was not statistically significant(t=-0.69,P>0.05).In the post-intervention cross-sectional comparison between groups,the sleep aid video group had better PSQI scores(4.90±0.74;6.30±0.67,5.80±0.79,6.20±0.63),sleep latency(0:25±0.24 h,1:31±0.36h,00:56±0.56 h,01:22±0.54h)and sleep efficiency[(81.83±5.40)%;(72.60±5.00)%,(75.50±4.43)%,(74.80±5.20)%]than the other 3 groups(F=8.00,6.61,19.00)and better sleep duration than the TV/movie strearming group and the online surfing/social networking group[(7.30±0.68)(6.20±0.26)(6.36±0.64)h](F=6.61)(P<0.01).Conclusions Bedtime screen use with different screen contents affects sleep quality differently.The screen content of TV/movie streaming and online surfing/socialising reduced sleep quality,whereas watc-hing sleep aiding videos may improve sleep quality,while playing video games shows no effects on sleep quality.