Drug resistance and virulence of 152 Salmonella strains
Objective This study was aimed at understanding the epidemic characteristics of drug resistance,the drug resistance genes,and the virulence island genes in foodborne Salmonella in Xuzhou from 2019 to 2021,to provide data support for the prevention and control of foodborne diseases in Xuzhou.Methods Mass spectrometry was used for bacterial identification and automatic microbial identification;a drug sensitivity analyzer was used for drug sensitivity testing;and PCR was used for screening drug resistance genes and virulence island genes.Results Only 4.6％of the 152 isolates were fully sensitive,whereas 82.9％were multi-drug resistant strains.The highest resistance rates were observed for ampicillin(78.3％),tetracycline(65.8％),ampicillin sulbactam(59.2％),cefazolin(53.3％),and chloramphenicol(50.7％).The resis-tance rates of the remaining six antibiotics were below 50.0％.Nine drug resistance genes were detected,among which the de-tection rates for the drug resistance genes gyrA,sul Ⅱ,and floR were 96.7％,85.5％,and 78.3％,respectively.A total of 17 virulence island genes were detected,among which the detection rate of the sipC,sopA,and sseL genes was 100.0％,and the detection rate of other virulence island genes exceeded 75.0％.Conclusion Many drug resistance genes and virulence is-land genes are carried by Salmonella in the Xuzhou area,and the drug resistance is severe;therefore,health supervision and management in this area should be strengthened.
SalmonellaDrug resistanceDrug resistance geneVirulence island gene
NETL
NSTL
万方数据
