Fingerprint of Guizhou Miao medicine Geum japonicum and determination of its main component ellagic acid
Objective:To establish the UHPLC fingerprint for Guizhou Miao medicine Geum japonicum and a method for determining its main component ellagic acid.Methods:The chromatographic separation was performed on the Phenomenex Synergi column (2.0 mm × 100 mm,2.5 μm) with gradient elution of methanol and 0.1％ aqueous formic acid at a flow rate of 0.3 mL· min-1.The column temperature was set at 40 ℃.Results:The UHPLC fingerprint with 17 common peaks was established for Geum japonicum.The method for determining ellagic acid in the same chromatographic condition was set up,which showed good linearity in the test ranges.The average recovery was 100.6％ and the RSD was 1.3％.Conclusion:The methods developed are convenient,rapid,accurate and reproducible,which is applicable to the quality control of Guizhou Miao medicine Geum japonicum.
NETL
NSTL
万方数据
维普
