Preparation and in vitro evaluation of resveratrol elastic liposomes
Objective:To prepare resveratrol elastic liposomes and investigate their in vitro properties.Methods:Liposomes were prepared by ethanol injection method.The formulation of resveratrol liposomes was optimized by single factor investigation and central composite design-response surface methodology with encapsulation efficiency and particle size as indexes.The ratio of surfactants was screened by skin retention;the surface characteristics,particle size,Zeta potential,in vitro release,and skin retention were determined.Results:The final prescription was as follows:the amount of soybean lecithin was7.5mg·mL-1,the mass ratio of phospholipid to cholesterol was 3.16∶1,the mass ratio of phospholipid to resveratrol was 30.28∶1,and the mass ratio of phospholipid to Tween-80 was3∶1.The average particle size of the prepared resveratrol elastic liposomes was(143.70±1.74)nm,the polydispersity index(PDI)was(0.092±0.060),and the Zeta potential was-(9.65±0.61)mV.In vitro release studies showed that resveratrol ethanol solution released 80%at 4 h,reaching release equilibrium.The cumulative release of resveratrol elastic liposomes at 8 h was 54%,and the release of resveratrol elastic liposomes continued slowly within 24 h,which was consistent with the Higuchi kinetic model.The results of skin retention test showed that its skin retention increased slowly at 2～6 h,proving that it could release slowly and reached 34.14 μg·cm-2 at 6 h.Conclusion:The optimized elastic liposomes had good reproducibility,high encapsulation rate and small particle size,obvious sustained release effect and high skin retention.
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