The chemical structure of an active polysaccharide RTP-60-3V-Ⅲ from roots of Ranunculus ternatus and its effect on drug-induced liver injury
Objective:To isolate and purify an active polysaccharide(RTP-60-3V-Ⅲ)from the roots of Ranunculus ternatus and investigate its antidrug-induced liver injury effects.Methods:The crude polysaccharides from the roots of R.ternatus were isolated and purified with graded alcohol precipitation,DEAE-cellulose column and Sephadex gel G100 and G50 columns to obtain the homogeneous polysaccharide.The molecular weight of RTP-60-3V-Ⅲ was determined with gel filtration chromatography and its monosaccharide composition was analyzed with capillary electrophoresis after acid hydrolysis and PMP derivation.The methods of periodate oxidation-Smith degradation,methylation analysis,FT-IR,NMR and GC-MS were used to investigate the chemical structural features of RTP-60-3V-Ⅲ.The effects of the polysaccharide on drug-induced liver injury in human LO2 liver cell model were estimated by MTT method using atorvastatin,dexamethasone,azithromycin,paclitaxel,and rifampicin.Results:RTP-60-3V-Ⅲ is a glucan and its peak height weight is 4 628 Da.Its backbone chain is →4,6)-α-D-Glcp-(1→ and with three branch chains of α-D-Glcp-(1→,→4)-α-D-Glcp-(1→and→6)-α-D-Glcp-(1→.The polysaccharide significantly reduced LO2 cell death induced by atorvastatin,dexamethasone,azithromycin,paclitaxel and rifampicin and exhibited good concentration-effect relations.Conclusion:The polysaccharide of RTP-60-3V-Ⅲ from Ranunculus ternatus possesses remarked effects on drug-induced liver injury,and it might be a good candidate in this field in future.
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