Optimization of extraction process of Jing Xuan Ning based on network pharmacology combined with multi index orthogonal test
Objective:To optimize the extraction process of Jing Xuan Ning by network pharmacology combined with multiple index orthogonal test.Methods:Network pharmacology combined with molecular docking was used to screen the potential active ingredients and targets of Jing Xuan Ning.The index components were identified according to the Chinese Pharmacopoeia(2020 edition).Using the information entropy weighting method and orthogonal test design,the optimal extraction process of Jing Xuan Ning was evaluated using transfer rate of the index ingredient,taking water addition times,extraction duration and extraction times as the influencing factors.Results:The index components were identified as gastrodin,4-hydroxybenzyl alcohol and puerarin.The optimum extraction method was to extract twice for 1.5 h each,adding 12 times of water the first time and 10 times of water the second time.Conclusion:By using network pharmacology and molecular docking techniques,the process evaluation indexes were determined,and the optimized extraction process of Jing Xuan Ning was reasonable,stable and well reproducible,which can provide a reference for subsequent in-depth research.
Jing Xuan Ningextraction processnetwork pharmacologymolecular dockinginformation entropy weighting methodorthogonal test
NETL
NSTL
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