Preparation process optimization of paeoniflorin PLGA nanoparticle and its protection of cardiomyocytes
Objective:To optimize the preparation process of paeoniflorin PLGA nanoparticles and to explore the protective effect of paeoniflorin nanoparticles on H2O2-injured H9c2 cardiomyocytes.Methods:Paeoniflorin PLGA nanoparticles were prepared by compound emulsion-solvent volatilization method,and Plackett-Burman design combined with Box-Behnken-response surface design was used to optimize the preparation process to obtain the optimum prescription.The surface features,4 ℃ storage situation and in vitro release of paeoniflorin PLGA nanoparticles prepared according to the best prescription were analyzed.The protective effect of paeoniflorin PLGA nanoparticles on H2O2-injured H9c2 cardiomyocytes was studied.The cell survival rate was measured by CCK-8 method.The amount of lactate dehydrogenase(LDH),malondialdehyde(MDA)and superoxide dismutase(SOD)were detected by commercial kits.Results:The optimized prescription is as follows:Poloxamer concentration 0.4％,paeoniflorin 3.1 mg,PLGA 21.4 mg,obtaining encapsulation efficiency is(45.49±0.29)％,loading efficiency is(4.52±0.05)％,particle size is(115.1±3.61)nm,polydiseperse index(PDI)is(0.122± 0.01),and Zeta potential is-(23.2±0.19)mV.It's regular and round in shape and the in vitro release was 80％in 12 h.The cell experiment results showed that paeoniflorin PLGA nanoparticles improved the survival rate of cardiomyocytes,reduced the LDH and MDA content,and increased the SOD content(P＜0.05).Conclusion:The optimized paeoniflorin PLGA nanoparticles were stable at 4 ℃ and released slowly in vitro.The rule of its release fits the Ritger-Peppas kinetic model.Paeoniflorin PLGA nanoparticles showed obvious protection against H2O2-induced damage in H9c2 cardiomyocytes.
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