Determination of index components in Jiawei Gegen Granules and multiple statistical analysis based on fingerprint map
Objective:To establish the fingerprint of Jiawei Gegen Granules,determine the contents of six components,and conduct orthogonal least squares criterion(OPLS-DA)analysis.Methods:The contents of six components were determined by HPLC with Tech Mate-C18 column applying the mobile phase of acetonitrile-0.05%phosphate solution in gradient elution.The flow rate was 1 mL·min-1,the detection wavelength was set at 254 nm,and the column temperature was 35℃.The similarity was evaluated with"TCM Chromatographic Fingerprint Similarity Evaluation System"(2012 Edition)by determining common peaks and the contents of six components.Finally,OPLS-DA analysis with SIMCA 14.0 software and the analytical variable importance projection(VIP)values were used to screen the markers.Results:There were 14 peaks in 10 batches of Jiawei Gegen Granules with similarity above 0.9.A total of six chromatographic peaks were identified and determined.The calibration curve of each component was linear in the test ranges with r values of 0.9990～0.9999.OPLS-DA screening obtained six common peaks(peaks 13,7,12,2,8,9)with variable importance projections(VIP)＞1,and three of them(cinnamic acid,protocatechuic acid,paeoniflorin)could be served as differential markers.Conclusion:Combined with multivariate statistical analysis,the established fingerprint map and content determination method are accurate,stable,and feasible,which can be used for the quality control of Jiawei Gegen Granules.
Jiawei Gegen Granulesfingerprintcontent determinationcluster analysis
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