基于指纹图谱的加味葛根颗粒6种指标成分含量测定及多元统计分析
Determination of index components in Jiawei Gegen Granules and multiple statistical analysis based on fingerprint map
李文莉 1李军鸽 2朱凯 2王永春 2楚云杰3
作者信息
- 1. 长春中医药大学附属医院,长春 130117;长春中医药大学,长春 130117
- 2. 长春中医药大学,长春 130117
- 3. 长春中医药大学附属医院,长春 130117
- 折叠
摘要
目的:建立加味葛根颗粒的指纹图谱,测定其中6 种成分的含量,并进行正交偏最小二乘判别法分析(OPLS-DA).方法:采用HPLC法,色谱柱为Tech Mate-C18柱,流动相为乙腈-0.05%磷酸溶液(梯度洗脱),检测波长为254 nm,柱温为35℃,流速为1mL·min-1.采用《中药色谱指纹图谱相似度评价系统》2012 年版进行相似度评价,确定共有峰,并测定其中6 种成分的含量;采用SIMCA 14.0 软件进行OPLS-DA分析,并利用分析变量重要性投影值(VIP)筛选影响其质量的标志物.结果:10 批加味葛根颗粒共有14 个共有峰,相似度均＞0.9;共指认了 6 个色谱峰并分别测定了含量,各成分在考察范围内线性良好,r 值在0.9990～0.999 9 之间.OPLS-DA筛选得到了6 个VIP＞1 的共有峰(峰13、峰7、峰12、峰2、峰8、峰9),经对照品指认了其中3 个差异性标志物(肉桂酸、原儿茶酸、芍药苷).结论:结合多元统计分析,建立的指纹图谱和含量测定方法准确、稳定、可行,可用于加味葛根颗粒的质量控制.
Abstract
Objective:To establish the fingerprint of Jiawei Gegen Granules,determine the contents of six components,and conduct orthogonal least squares criterion(OPLS-DA)analysis.Methods:The contents of six components were determined by HPLC with Tech Mate-C18 column applying the mobile phase of acetonitrile-0.05%phosphate solution in gradient elution.The flow rate was 1 mL·min-1,the detection wavelength was set at 254 nm,and the column temperature was 35℃.The similarity was evaluated with"TCM Chromatographic Fingerprint Similarity Evaluation System"(2012 Edition)by determining common peaks and the contents of six components.Finally,OPLS-DA analysis with SIMCA 14.0 software and the analytical variable importance projection(VIP)values were used to screen the markers.Results:There were 14 peaks in 10 batches of Jiawei Gegen Granules with similarity above 0.9.A total of six chromatographic peaks were identified and determined.The calibration curve of each component was linear in the test ranges with r values of 0.9990～0.9999.OPLS-DA screening obtained six common peaks(peaks 13,7,12,2,8,9)with variable importance projections(VIP)＞1,and three of them(cinnamic acid,protocatechuic acid,paeoniflorin)could be served as differential markers.Conclusion:Combined with multivariate statistical analysis,the established fingerprint map and content determination method are accurate,stable,and feasible,which can be used for the quality control of Jiawei Gegen Granules.
关键词
加味葛根颗粒/指纹图谱/含量测定/聚类分析Key words
Jiawei Gegen Granules/fingerprint/content determination/cluster analysis引用本文复制引用
基金项目
吉林省科技发展计划项目(20220401063YY)
出版年
2024
NETL
NSTL
万方数据