Matrix effects in liquid chromatography-mass spectrometry analysis techniques
Liquid chromatography-mass spectrometry(LC-MS)technology is widely applied to the detection of trace amounts of substances due to its high sensitivity and specificity.However,matrix effects could occur in the method of LC-MS,especially for complex matrices,which could seriously affect the reliability of the results,manifested as ion suppression or ion enhancement.In order to overcome this interference,matrix effects should be examined before mass spectrometry analysis to ensure the accuracy of the results.The investigation of matrix effects is mainly divided into two parts:evaluation and overcoming.Currently,the commonly used evaluation methods are the post-column infusion,post-extraction spike,and slope analysis.Overcoming matrix effects includes reducing matrix effects and compensating for matrix effects.Reducing matrix effects can be achieved through preprocessing methods and optimizing the conditions of chromatography and mass spectrometry.Compensating for matrix effects is the elimination of interference from matrix effects through appropriate calibration methods.The mechanism,evaluation,elimination/compensation of matrix effects were summarized in this review,attempting to establish the respective guiding methods.
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