液质联用分析技术中的基质效应
Matrix effects in liquid chromatography-mass spectrometry analysis techniques
冯媛媛 1田冶 1尹利辉 1宁保明1
作者信息
- 1. 中国食品药品检定研究院,北京 102629
- 折叠
摘要
液质联用技术因超高的灵敏度和特异性而广泛应用于微量、痕量物质的检测.但是液质联用会产生基质效应,尤其在复杂基质下会严重影响结果的可靠性,具体表现为离子抑制或离子增强现象.为了克服这种干扰,在进行质谱分析前,要对基质效应进行考察来保证结果的准确性.考察基质效应主要分为评价和克服两部分,目前常用的评价方法为柱后注射法、提取后添加法和斜率分析法.克服基质效应分为减小基质效应和补偿基质效应,减小基质效应可以通过前处理方法、优化色谱和质谱条件来实现,补偿基质效应通过合适的校准方法消除基质效应的干扰.本文对基质效应的机制、评价、消除/补偿进行综述,尝试建立发现、消除/补偿基质效应的方法.
Abstract
Liquid chromatography-mass spectrometry(LC-MS)technology is widely applied to the detection of trace amounts of substances due to its high sensitivity and specificity.However,matrix effects could occur in the method of LC-MS,especially for complex matrices,which could seriously affect the reliability of the results,manifested as ion suppression or ion enhancement.In order to overcome this interference,matrix effects should be examined before mass spectrometry analysis to ensure the accuracy of the results.The investigation of matrix effects is mainly divided into two parts:evaluation and overcoming.Currently,the commonly used evaluation methods are the post-column infusion,post-extraction spike,and slope analysis.Overcoming matrix effects includes reducing matrix effects and compensating for matrix effects.Reducing matrix effects can be achieved through preprocessing methods and optimizing the conditions of chromatography and mass spectrometry.Compensating for matrix effects is the elimination of interference from matrix effects through appropriate calibration methods.The mechanism,evaluation,elimination/compensation of matrix effects were summarized in this review,attempting to establish the respective guiding methods.
关键词
基质效应/机制/评价/清除Key words
matrix effects/mechanism/evaluation/overcome引用本文复制引用
出版年
2024
NETL
NSTL
万方数据