Preparation and in vitro evaluation of three nicotine transdermal vesicles
Objective:To prepare nicotine-ethanol liposomes,diol liposomes and transporters,compare their skin permeability,screen the best transdermal transport vesicles and optimize the transdermal transport of drugs.Methods:Nicotine-ethanol liposomes and diol liposomes were prepared by injection method,and the transfer bodies were prepared by thin film dispersion method.Based on the morphology,size of particle size,zeta potential value and encapsulation rate,the prescriptions of three kinds of vesicles were optimized and screened by Franz diffusion cell,and confocal laser scanning electron microscopy(CLSM)was used to compare the transdermal depth of three lipid vesicles.Results:When ethanol:propylene glycol=5:5(w/w),the diol liposome was the most stable,the encapsulation rate was the highest(86.13±1.40)％,the particle size was the smallest(106.00±11.0)nm,the transdermal depth was the largest(180 μm).The 24 h cumulative penetration percentages of the ethanol liposome,diol liposome(ethanol:propylene glycol=5:5,w/w)and transporters in the skin were 4.07(P<0.05),8.62(P<0.01)and 2.83(P>0.05)times of that of the standard liposomes,respectively.The CLSM experiment showed that the penetration depth and fluorescence intensity of Rhodamine B in ethanol:propylene glycol=5:5(w/w)diol liposomes were much higher than those in ethanol liposomes and transporters.Conclusion:Diol liposomes(ethanol:propylene glycol=5:5,w/w)carry drugs through the skin more efficiently than ethanol liposomes and transporters,which significantly increase the dermal retention of drugs.
nicotineethanol liposomediol liposomesconfocal laser scanning electron microscopepercutaneous transport
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