Advances in the co-culture models for evaluating gastrointestinal toxicity in vitro
In recent years,in vitro substitution tests have become increasingly important in gastrointestinal toxicity studies.Traditional non-clinical studies of new drugs are mainly based on animal experiments.However,with the development of biotechnology and the requirements of animal welfare,in vitro gastrointestinal model has gradually shifted from in vivo tests to in vitro,from a single cell type to an organoid co-culture model that is closer to the in vivo environment.It can even be used for multi-organ fusion culture and microfluidic culture.By simulating the physiological and pathological environment consistent with in vivo,these culture systems can be used not only for the modeling of gastrointestinal diseases,but also as a platform for drug toxicity screening,which promotes the progress of in vitro toxicity evaluation.In this paper,the research progress of conventional in vitro gastrointestinal model,gastrointestinal organoid model and microfluidic chip are reviewed.
gastrointestinal toxicityorganoidsculture in vitroco-culturemicrofluidic chip
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