超高效液相色谱-四极杆-飞行时间质谱分析匙羹藤化学成分
Analysis of chemical constituents in Gymnema sylvestre(Retz.)Schult via ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry
曾志浩 1肖观林 2贾灿潮 1江洁怡 2汤瑞茵 1贾德政 1李养学 2李绍林 3毕晓黎4
作者信息
- 1. 广州中医药大学第五临床医学院,广州 510405
- 2. 广东省中医药工程技术研究院,广州 510095;广东省中医药研究开发重点实验室,广州 510095
- 3. 广东食品药品职业学院,广州 510520
- 4. 广州中医药大学第五临床医学院,广州 510405;广东省中医药工程技术研究院,广州 510095;广东省中医药研究开发重点实验室,广州 510095
- 折叠
摘要
目的:基于超高效液相色谱-四极杆-飞行时间质谱(ultra-performance liquid chromatography qua-drupole time-of-flight mass spectrometry,UPLC-Q-TOF-MS/MS)技术分析匙羹藤的化学成分.方法:采用 Wa-ters CORTECS UPLC T3色谱柱(150 mm×2.1 mm,1.6 μm)以0.1％甲酸-乙腈的流动相梯度洗脱,电喷雾负离子模式下对色谱流出物进行检测,检测范围m/z50～2000.结果:通过高分辨质谱数据(一级质谱及二级质谱碎片)分析,结合数据库、文献以及对照品比对,共鉴别出101种成分,包括53种三萜类、37种黄酮类、5种有机酸类、3种单萜类、1种二萜类、1种菲类、1种甾体类化合物.结论:本研究通过UPLC-Q-TOF-MS/MS技术快速鉴别了匙羹藤的化学成分,该方法快速、准确、简便,能够用于匙羹藤化学成分的定性鉴定,为该草药的质量标准以及物质基础研究奠定基础.
Abstract
Objective:Ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS)was used to analyze the chemical constituents of Gymnema sylvestre(Retz.)Schult.Methods:A Waters CORTECS UPLC T3(150 mm ×2.1 mm,1.6 μm)column was utilized to separate the components using the mobile phase at a gradient elution of 0.1％formic acid-acetonitrile and the effluent was detected in electrospray anionic mode with the detection range of m/z 50～2 000.Results:Combined with the databases,literature,and reference standards,we identified 101 components,including 53 triterpenoids,37 flavonoids,5 organic acids,3 monoterpenoids,1 diterpenoid,1 phenanthrene,and 1 steroid based on the analysis of high-resolution mass spectrometry data(primary mass spectra and secondary mass spectral fragments).Conclusion:The chemical constituents of Gymnema sylvestre were identified using the fast,accurate,and simple UPLC-Q-TOF-MS/MS method for qualitative identification,which laid the foundation for establishing a quality standard of the herb and its material basis research.
关键词
匙羹藤/超高效液相色谱-四极杆-飞行时间质谱/化学成分/三萜类化合物/黄酮类化合物Key words
Gymnema sylvestre/ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry/chemical constituents/triterpenoids/flavonoids引用本文复制引用
基金项目
广东省科技创新战略专项(部省市联动专项与省属科研机构稳定性支持)资助项目(粤财科教[2021]113号)
广州市科技计划资助项目(202102080588)
出版年
2024
NETL
NSTL
万方数据