Study on mechanism of Coptidis Rhizoma in the treatment of arthritis based on network pharmacology and molecular docking
Objective:To explore the mechanism of Coptidis Rhizoma(CR)in the treatment of arthritis based on network pharmacology and molecular docking.Methods:The chemical constituents and related targets of CR in TCMSP database were searched,and the targets were normalized and standardized by Uniprot database.The targets of rheumatoid arthritis,osteoarthritis and gouty arthritis were searched in GeneCards,OMIM and other databases.Venny website was used to obtain the intersection targets of CR and three kinds of arthritis,the STRING database was used to construct the protein-protein interaction(PPI)network of the intersection targets,and the PPI network was constructed by Cytoscape software.The common intersection targets were imported into DAVID database and Metascape database for bioinformatics analysis,and the related pathways in the treatment of arthritis were obtained.The chemical components,common targets and pathways corresponding to the three types of arthritis were imported into Cytoscape software to construct a network topology diagram of"chemical composition-disease-target-pathway".Results:A total of 14 chemical constituents in CR and 176 potential targets of arthritis were screened out.The related targets of regulating three kinds of arthritis were 5704,4098 and 738 respectively,and a total of 29 intersection targets were screened out.KEGG results showed that the treatment of arthritis with CR was related to pathways in cancer,AGE-RAGE signaling pathway in diabetic complications,Hepatitis B and other pathways.Conclusion:Berberine,palmatine,coptisine,berlambine,and other core components in CR may play an anti-arthritic role by regulating interleukin-17 signaling pathway and tumor necrosis factor signaling pathway to improve inflammation.
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