Optimization of enzymatic extraction of polysaccharides from Dendrobium officinale and the antioxidant activity
Objective:To explore the optimal extraction process of five enzyme extraction methods for Dendrobium officinale polysaccharides(DOP)by using orthogonal method,and the effects of enzyme extraction on molecular weight,viscosity,and antioxidant activity of DOPs.Methods:Orthogonal experiments were conducted on polysaccharides enzyme extraction by using cellulase,pectinase,amylase,mannanase,and glucanase,respectively.Meanwhile,DOP was extracted by using traditional water extraction method.The yield,molecular weight,viscosity,and in vitro antioxidant capacity of polysaccharides from different extraction processes were evaluated.Results:Five optimal extraction processes for enzyme extraction method were obtained.The yield of polysaccharides obtained by different extraction methods were as follows:glucosidase(55.41％)＞pectinase(53.12％)＞cellulase(51.93％)＞mannanase(48.42％)＞amylase(46.90％)＞water extraction(38.76％).Compared with traditional hot water extraction methods,the molecular weight and viscosity of polysaccharides obtained by enzyme extraction methods significantly reduced,and amylase had a slightly weaker effect on reducing molecular weight.Polysaccharides extracted by different methods exhibited different antioxidant abilities.Polysaccharides obtained by pectinase had the strongest antioxidant activity and overall superior to polysaccharides obtained by using traditional water extraction methods.Conclusion:Enzyme extraction methods significantly improved the extraction rate of polysaccharides,and the physicochemical properties such as molecular weight and viscosity significantly reduced.The antioxidant activity of polysaccharides extracted by pectinase is the most significant.
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