目的:建立一种全面、无创、快速、准确评估动物胃肠功能损伤的血清学方法。方法:构建SD大鼠胃肠损伤模型后，采集血液检测胃蛋白酶原(pepsinogen，PG，分为 2 个亚型:PGⅠ和PGⅡ)、胃泌素(gastrin 17，G-17)、瓜氨酸(citrulline，Cit)、肠型脂肪酸结合蛋白(intestinal type fatty acid binding protein，I-FABP)等血清学指标，同时进行胃、肠等组织病理学检查，探讨病理结果与血清学检测结果的相关性。结果:与对照组相比，模型组动物血清学结果提示:血清PGⅠ水平下降、PGⅡ水平升高(P<0。05)，PGⅠ/PGⅡ比值降低(P<0。05)，血清G-17 水平升高(P<0。01)，血清Cit水平下降(P<0。05)，血清I-FABP水平下降(P<0。05)。结论:PGⅠ，PGⅡ，G-17，Cit，I-FABP等指标可试用于药物非临床评价中胃肠功能损伤的早期诊断。
Study on application of new clinical pathology markers in assessment of drug-induced non-clinical gastrointestinal dysfunction
Objective:To establish a comprehensive,non-invasive,rapid and accurate serological method for evaluating gastrointestinal dysfunction in animals.Methods:After establishing the model of gastrointestinal injury in SD rats,blood samples were collected to detect the serum levels of pepsinogen(PG),gastrin(G-17),citrulline(Cit)and intestinal fatty acid binding protein(I-FABP),at the same time,the histopathology of stomach and intestines were examined to explore the correlation between the pathological results and the serological results.Results:Compared with the control group,the serum levels of PGⅠ,Cit,I-FABP and PGⅠ/PGⅡ ratio were decreased(P<0.05),while serum levels of PGⅡ(P<0.05)and G-17(P<0.01)were increased in the model group.Conclusion:PG,G-17,Cit,I-FABP can be used in the early diagnosis of gastrointestinal dysfunction in non-clinical evaluation of drugs.
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