目的 探究心房颤动(简称房颤)患者左房心肌中胶原蛋白(Collagen)、弹性蛋白(Elastin)的表达水平以及调控弹性蛋白生成的基质金属蛋白酶(MMPs)、金属蛋白酶组织抑制剂(TIMPs)蛋白表达水平和纤维化程度。方法 收集房颤患者和窦性心律患者左房组织标本，用HE、Masson染色检测心肌纤维化，用免疫荧光法和蛋白免疫印迹法检测Collagen Ⅰ、Collagen Ⅲ、Elastin、MMP-2、MMP-9和TIMP-2的蛋白表达变化。结果 与对照组(n=5)相比，房颤组(n=10)Collagen Ⅰ、Collagen Ⅲ 蛋白表达明显上调(0。42±0。17 vs 0。05±0。04，1。10±0。73 vs 0。23± 0。15，P 均＜0。05)，Elastin 蛋白水平下降(0。47±0。27 vs 2。17±1。05，P＜0。05);MMP-2、MMP-9 蛋白表达增加，TIMP-2蛋白表达减少。结论 房颤左房弹性蛋白水平下降，胶原蛋白水平增加，引起纤维化程度加重，这一过程可能受MMP-2、MMP-9和TIMP-2的共同调控。
Expression of collagen and elastin in patients with atrial fibrillation
Objective To investigate the expression levels of collagen and elastin,matrix metalloproteinases(MMPs)and tissue inhibitors of metalloproteinases(TIMPs),and the degree of fibrosis in atrial fibrillation pa-tients.Methods Left atrial tissue samples from patients with atrial fibrillation and sinus rhythm were collected,myocardial fibrosis was detected by HE and Masson staining,and protein expression of Collagen I,Collagen Ⅲ,E-lastin,MMP-2,MMP-9 and TIMP-2 were detected by immunofluorescence and western blotting.Results Com-pared with the control group,the protein expressions of Collagen Ⅰ and Collagen Ⅲ in AF group were significantly up-regulated,and the level of Elastin protein was decreased.The expression of MMP-2 and MMP-9 protein in-creased,while the expression of TIMP-2 protein decreased.Conclusion The decreased ratio of elastin/collagen in left atrium under atrial fibrillation leads to the aggravation of fibrosis,which may be co-regulated by MMP-2,MMP-9 and TIMP-2.[Chinese Journal of Cardiac Pacing and Electrophysiology,2024,38(2):90-95]
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