The effect of AMPA receptors on the susceptibility to atrial fibrillation in anxious rats
Objective To explore the effect of AMPA receptors on the susceptibility to atrial fibrillation(AF)in anxiety rats.Methods Sprague-Dawley(SD)rats of 6-8 weeks were used to establish anxiety rat model by empty bottle stimulation.Rats were randomly divided into control group(CTL group),model group(AD group),AMPA receptor antagonist(NBQX)group(ADN group)and ADN+PI3K inhibitor(LY294002)group(ADN+LY group).After the 21-day stimulation of empty bottle stimulation,the rats in each group were examined the frequen-cy of central entries,the cumulative time in the center,and the number of rearing by open field test and AF induc-ibility by electrophysiological test.The left atrial tissue was taken for apoptosis detection.The expression of GRIA1,Caspase-3,Cleaved caspase-3,Bax,Bcl-2,PI3K,p-PI3K,AKT and p-AKT in myocardial tissue was detected by Western blot.Results Compared with CTL group,the expression of anxiety behavior related indexes in AD group decreased;the induction rate of AF was increased,myocardial apoptosis was increased significantly,the ex-pression of GRIA1,Caspase-3,Cleaved caspase-3 and Bax were increased,while the expression of Bcl-2,p-PI3K/PI3K and p-AKT/AKT was decreased.Compared with AD group,the induction rate of AF and apoptosis in ADN group were decreased,the expression of GRIA1,Caspase-3,Cleaved caspase-3 and Bax in atrial tissue were de-creased,while the expression of Bcl-2,p-PI3K/PI3K and p-AKT/AKT was increased in ADN group.Compared with ADN group,theinduction rate of AF and myocar-dial apoptosis were increased significantly;the expres-sion of GRIA1,Caspase-3,Cleaved caspase-3 and Bax was increased and the expression of Bcl-2,p-PI3K/PI3K and p-AKT/AKT was decreased in ADN+LY group.Conclusion The inhibition of AMPA receptors can im-prove myocardial cell apoptosis and reduce the inducibility of atrial fibrillation through PI3K/AKT pathway in anxiety rats.[Chinese Journal of Cardiac Pacing and Electro-physiology,2024,38(3):180-186]
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