冠心病患者ABCG1、ANGPTL2启动子区甲基化与心力衰竭发生的关系研究
Relationship between promoter methylation of ABCG1 and ANGPTL2 and heart failure in patients with coronary heart disease
颜春晖 1游三丽 1徐勤 1袁李礼 1王朝华1
作者信息
- 1. 410007 长沙,湖南省脑科医院(湖南省第二人民医院)心血管内科二区
- 折叠
摘要
目的 分析冠状动脉粥样硬化性心脏病(冠心病)患者三磷酸腺苷结合盒转运蛋白G1(ABCG1)、血管生成素样蛋白 2(ANGPTL2)启动子区甲基化与心力衰竭(心衰)发生的关系.方法 选取2020年3月至2022年3月于湖南省第二人民医院收治的冠心病患者120例.根据是否发生心衰,将患者分为合并心衰组(n=26)和不合并心衰组(n=94).采用全自动生化仪检测血清总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C).采用心脏超声检查患者左室射血分数(LVEF)、左心室舒张末期内径(LVEDD)、左室收缩末期内径(LVESD)水平.特异性PCR检测ABCG1和ANGPTL2基因甲基化.采用实时荧光定量聚合酶链反应检测ABCG1和ANGPTL2基因mRNA表达水平.Logistic回归分析探讨影响冠心病患者发生心衰的因素.结果 两组患者LDL-C、LVEF、LVEDD、LVESD、ABCG1、ANGPTL2基因启动子区甲基化等方面比较,差异具有统计学意义(P＜0.05).LVESD、LVEF、ABCG1甲基化和ANGPTL2甲基化是冠心病患者发生心衰的危险因素.ABCG1甲基化、ANGPLT2甲基化患者中LVESD水平均高于未甲基化患者(P＜0.05).ABCG1甲基化、ANGPLT2甲基化患者中LVEF水平均低于未甲基化患者(P＜0.05).合并组ABCG1、ANGPTL2基因甲基化的患者mRNA的表达量明显低于非甲基化患者(P＜0.05).结论 ABCG1和ANGPTL2基因甲基化是冠心病患者发生心衰的危险因素,检测上述两基因甲基化状态可为诊治冠心病心衰提供理论支持.
Abstract
Objective To analyze the relationship between promoter methylation of adenosine triphophate(ATP)-binding cassette(ABC)transporter G1(ABCG1)and angiopoietin-like protein 2(ANGPTL2)and heart failure in patients with coronary heart disease(CHD).Methods A total of 120 patients with CHD admitted to the hospital were enrolled between March 2020 and March 2022.According to the presence or absence of heart failure,they were divided into a combination group(n=26)and a CHD group(n=94).The levels of serum total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),and high-density lipoprotein cholesterol(HDL-C)were detected by full-automatic biochemical analyzer.The left ventricular ejection fraction(LVEF),left ventricular end-diastolic diameter(LVEDD),and left ventricular end-systolic diameter(LVESD)were detected by echocardiography.The methylation of ABCG1 and ANGPTL2 genes was detected by specific PCR.The mRNA levels of ABCG1 and ANGPTL2 genes were detected by real-time fluorescence quantitative polymerase chain reaction.The influencing factors of heart failure were analyzed by Logistic regression analysis.Results There were significant differences in LDL-C,LVEF,LVEDD,LVESD,promoter methylation of ABCG1 and ANGPTL2 genes between the two groups(P<0.05).LVESD,LVEF,methylation of ABCG1 and ANGPTL2 were risk factors for heart failure.LVESD in patients with methylation of ABCG1 and ANGPLT2 was higher than that without methylation(P<0.05),while LVEF was lower than that without methylation(P<0.05).The mRNA levels of ABCG1 and ANGPTL2 genes in patients with methylation of ABCG1 and ANGPLT2 were significantly lower than those without methylation(P<0.05).Conclusion The methylation of ABCG1 and ANGPTL2 genes is a risk factor of heart failure in CHD patients.Detection of the methylation status can provide scientific support for the diagnosis and treatment of heart failure in coronary heart disease.
关键词
冠心病/三磷酸腺苷结合盒转运蛋白G1/血管生成素样蛋白2/心力衰竭Key words
Coronary Heart Disease/Adenosine Triphosphate Binding Cassette Transporter G1/Angiopoietin-Like Protein 2/Heart Failure引用本文复制引用
基金项目
湖南省卫生健康委科研项目(C2019042)
出版年
2024
NETL
NSTL
维普
万方数据